Daripally Sarika, Nallapalle Sateesh Reddy, Katta Saritha, Prasad Vidudala V T S
Research and Development, Basavatarakam Indo-American Cancer Hospital and Research Institute, Road No. 10, Banjara Hills, Hyderabad, 500034, India.
Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
Tumour Biol. 2015 Sep;36(10):7817-30. doi: 10.1007/s13277-015-3516-x. Epub 2015 May 6.
We investigated risk association of oral cancers (tongue and buccal mucosa cancers) with FAS (-1377G > A and FAS -670 A > G) and FASL (-844 T > C) SNPs, in males and females. A case-control study of 535 oral cancer and 525 control subjects was performed. SNPs were detected in the genomic DNA isolated from peripheral blood using PCR-RFLP. We report FASL -844 T > C SNPs increased risk for buccal mucosa cancer in females but not in males. On the other hand, FAS genotypes did not alter the risk of the cancers in both females and males. However, co-occurrence of FAS -1377 GA and -670 GG, FAS -1377 AA and -670 GG genotypes, and combined genotypes of FAS and FASL (FAS -1377 AA + FAS -670 GG + FASL -844 CC) alter male susceptibility towards tongue cancer. In females, combined genotypes of FAS (-1377GA and -670 AA) were found to be a risk factor of buccal mucosa cancer (OR = 3.27, CI = 1.28-8.36; P ≤ 0.01). FASL variants (GA and AA) increased tongue cancer risk in females who were tobacco users compared to non-tobacco users. In conclusion, SNPs of the FAS and FASL might alter risk of tongue and buccal mucosa cancers differentially, in a gender-dependent manner.
我们调查了男性和女性口腔癌（舌癌和颊黏膜癌）与FAS基因（-1377G＞A和FAS -670 A＞G）及FASL基因（-844 T＞C）单核苷酸多态性（SNP）之间的风险关联。开展了一项病例对照研究，纳入535例口腔癌患者和525例对照者。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测外周血分离的基因组DNA中的SNP。我们报告，FASL -844 T＞C SNP增加了女性颊黏膜癌的风险，但男性未出现此情况。另一方面，FAS基因的基因型并未改变女性和男性患癌风险。然而，FAS -1377 GA和-670 GG、FAS -1377 AA和-670 GG基因型同时出现，以及FAS和FASL的联合基因型（FAS -1377 AA + FAS -670 GG + FASL -844 CC）改变了男性对舌癌的易感性。在女性中，发现FAS联合基因型（-1377GA和-670 AA）是颊黏膜癌的一个风险因素（比值比[OR]=3.27，置信区间[CI]=1.28 - 8.36；P≤0.01）。与非烟草使用者相比，FASL变异型（GA和AA）增加了女性烟草使用者患舌癌的风险。总之，FAS和FASL的SNP可能以性别依赖的方式不同程度地改变舌癌和颊黏膜癌的风险。
