Joshua G W, Harrison L J, Sewell M M
Department of Tropical Animal Health, University of Edinburgh, Easter Bush, Roslin, Midlothian.
Parasitology. 1989 Oct;99 Pt 2:265-74. doi: 10.1017/s0031182000058728.
Direct surface 125I radio-isotope labelling techniques and SDS-PAGE analysis were used to compare the proteins and lentil-lectin adherent glycoproteins of the bovine stage of viable Taenia saginata larvae at three points in their development, the invasive oncospheres, immature (4-week-old) and mature (12 to 16-week-old) cysticerci. Some proteins and glycoproteins were present on all three of the ages of the parasite examined but there were also distinct age-specific proteins and glycoproteins detected on oncospheres and 4-week-old cysticerci and a marked difference between the protein/glycoprotein profiles of the parasite was apparent at these earlier stages of development and the mature cysticeri. The latter were characterized by the presence of high, 160-200 kDa molecular weight, lysine rich, glycoproteins, whereas small 16 and 18 kDa glycoproteins and a reduction-sensitive 23 kDa glycoprotein were first detected on 4-week-old immature cysticerci. Antigenic characterization of the isotope-labelled proteins and glycoproteins by immunoprecipitation against a panel of clinically defined bovine sera combined with SDS-PAGE analysis indicated that relatively few proteins were precipitated by sera from T. saginata-infected cattle as compared to the glycoproteins. However, both protein and glycoprotein antigens of possible protective and/or diagnostic significance were identified from oncospheres and cysticerci. Of particular note were low molecular weight oncospheral proteins and low and high molecular weight cysticercal glycoproteins. The immunogenicity of these protein and glycoprotein antigens of T. saginata larvae and their age-related changes are of relevance to the design of diagnostic assays, vaccines and possibly to the survival of this parasite in its bovine host.
采用直接表面¹²⁵I放射性同位素标记技术和SDS-PAGE分析，比较了牛带绦虫活幼虫牛期发育三个阶段（侵入性六钩蚴、未成熟（4周龄）和成熟（12至16周龄）囊尾蚴）的蛋白质和扁豆凝集素粘附糖蛋白。在所检测的寄生虫的所有三个年龄阶段均存在一些蛋白质和糖蛋白，但在六钩蚴和4周龄囊尾蚴上也检测到了明显的年龄特异性蛋白质和糖蛋白，并且在发育的这些早期阶段与成熟囊尾蚴之间，寄生虫的蛋白质/糖蛋白谱存在明显差异。后者的特征是存在高分子量（160 - 200 kDa）、富含赖氨酸的糖蛋白，而小分子量的16 kDa和18 kDa糖蛋白以及一种对还原敏感的23 kDa糖蛋白首次在4周龄未成熟囊尾蚴上检测到。通过针对一组临床定义的牛血清进行免疫沉淀结合SDS-PAGE分析，对同位素标记的蛋白质和糖蛋白进行抗原特性分析，结果表明，与糖蛋白相比，来自牛带绦虫感染牛的血清沉淀的蛋白质相对较少。然而，从六钩蚴和囊尾蚴中鉴定出了具有潜在保护和/或诊断意义的蛋白质和糖蛋白抗原。特别值得注意的是低分子量的六钩蚴蛋白以及低分子量和高分子量的囊尾蚴糖蛋白。牛带绦虫幼虫这些蛋白质和糖蛋白抗原的免疫原性及其与年龄相关的变化与诊断检测、疫苗的设计以及该寄生虫在其牛宿主中的存活可能都有关系。
