Joshua G W, Harrison L J, Sewell M M
University of Edinburgh, Department of Tropical Animal Health, Roslin, Midlothian, Scotland.
Parasitology. 1990 Jun;100 Pt 3:463-7. doi: 10.1017/s003118200007877x.
Taenia saginata cyst fluid proteins from 4, 8, 12 and 16-week-old cysticerci were analysed by a combination of direct 125I radio-isotope labelling, immunoprecipitation using a panel of sera from infected cattle infected with T. saginata and SDS-PAGE. Protein antigens of 12, 14, 16, 20 and 26 kDa were identified in all of the cyst fluids examined. These were immunogenic and were precipitated by serum taken from cattle from 8 weeks after infection onwards and were therefore considered to be of diagnostic potential. A 185 kDa protein antigen found only in the cyst fluid of 4-week-old cysticerci and a 43 kDa protein antigen first detected in cyst fluid from 8-week-old cysticerci were also identified but were considered to be of more limited diagnostic potential due to their restricted presence. An apparently non-immunogenic 67 kDa protein, found in all the cyst fluids examined, may have been host serum albumin.
采用直接¹²⁵I放射性同位素标记、使用一组感染牛带绦虫的牛血清进行免疫沉淀以及SDS - PAGE相结合的方法，对4周龄、8周龄、12周龄和16周龄囊尾蚴的牛带绦虫囊液蛋白进行了分析。在所检测的所有囊液中均鉴定出了12 kDa、14 kDa、16 kDa、20 kDa和26 kDa的蛋白质抗原。这些抗原具有免疫原性，从感染后8周起采集的牛血清可使其沉淀，因此被认为具有诊断潜力。还鉴定出了仅在4周龄囊尾蚴的囊液中发现的185 kDa蛋白质抗原以及首次在8周龄囊尾蚴的囊液中检测到的43 kDa蛋白质抗原，但由于它们存在的局限性，其诊断潜力被认为较为有限。在所有检测的囊液中均发现的一种明显无免疫原性的67 kDa蛋白质，可能是宿主血清白蛋白。
