中性粒细胞分布在胆管癌中的预后价值。
Prognostic value of neutrophil distribution in cholangiocarcinoma.
作者信息
Mao Zhi-Yuan, Zhu Guang-Qing, Xiong Mei, Ren Li, Bai Li
机构信息
Zhi-Yuan Mao, Guang-Qing Zhu, Department of Oncology, Air Force General Hospital of PLA, Beijing 100142, China.
出版信息
World J Gastroenterol. 2015 Apr 28;21(16):4961-8. doi: 10.3748/wjg.v21.i16.4961.
AIM
To explore the relationship of clinicopathological features and the distribution of neutrophils in the tumor microenvironment with the prognosis of cholangiocarcinoma.
METHODS
Two hundred and fifty-four formalin-fixed and paraffin embedded tissue blocks were analyzed, including tissues from cholangiocarcinoma (n = 254), and tumor adjacent tissues (n = 238). Tissue sections were stained for CD15 using immunohistochemical staining. CD15 expression was detected to identify the distribution of neutrophils in the local tumor microenvironment. The neutrophil density of the tumor tissues and the adjacent tumor tissues was detected to reflect their inflammatory status. Clinical data and follow-up information of cholangiocarcinoma patients who underwent surgery from January 2004 to December 2010 were analyzed retrospectively. The relationship between clinicopathological features and the distribution of neutrophils with prognosis of the patients were analyzed.
RESULTS
The positive expression level of CD15 was only significantly related to the TNM stage. CD15 expression was higher in tumor tissues than in adjacent tissues (73.6% vs 54.6%), with significant differences. Patients with high expression of CD15 had significantly shorter overall survival (OS) than those with low expression of CD15 (median overall survival time 39.77 mo vs 16.87 mo, P = 0.008). Patients with high CD15 expression had significantly shorter disease free survival time (DFS) than those with low expression of CD15 (median DFS 38.27 mo vs 16.83 mo, P = 0.029). COX multivariate analysis indicated that high CD15 expression in tumor tissues was an independent risk factor for predicting OS for patients with cholangiocarcinoma [P = 0.012, relative risk (RR) = 1.601], but it was not an independent risk factor for predicting DFS (P = 0.073, RR = 1.462).
CONCLUSION
Patients with high CD15 expression in cancer tissues had shorter DFS and OS. High expression of CD15 is an independent risk factor for OS.
目的
探讨胆管癌的临床病理特征及肿瘤微环境中中性粒细胞的分布与预后的关系。
方法
分析254个福尔马林固定石蜡包埋组织块，包括胆管癌组织（n = 254）和癌旁组织（n = 238）。采用免疫组织化学染色法对组织切片进行CD15染色。检测CD15表达以确定局部肿瘤微环境中中性粒细胞的分布。检测肿瘤组织和癌旁组织的中性粒细胞密度以反映其炎症状态。回顾性分析2004年1月至2010年12月接受手术的胆管癌患者的临床资料和随访信息。分析临床病理特征及中性粒细胞分布与患者预后的关系。
结果
CD15的阳性表达水平仅与TNM分期显著相关。肿瘤组织中CD15表达高于癌旁组织（73.6%对54.6%），差异有统计学意义。CD15高表达患者的总生存期（OS）明显短于CD15低表达患者（中位总生存时间39.77个月对16.87个月，P = 0.008）。CD15高表达患者的无病生存期（DFS）明显短于CD15低表达患者（中位DFS 38.27个月对16.83个月，P = 0.029）。COX多因素分析表明，肿瘤组织中CD15高表达是预测胆管癌患者OS的独立危险因素[P = 0.012，相对危险度（RR）= 1.601]，但不是预测DFS的独立危险因素（P = 0.073，RR = 1.462）。
结论
癌组织中CD15高表达的患者DFS和OS较短。CD15高表达是OS的独立危险因素。
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