Research center for Infectious Diseases of Digestive System, Physiology Research Center, and Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Physiology Research Center, and Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Basic Med Sci. 2015 Mar;18(3):253-8.
This study was undertaken to investigate the protective effects of vitamin B6, cofactor for cystathionine-γ lyase and cystathionine-β synthase (producers of H2S), alone and in combination with L-cysteine, H2S precursor, on indomethacin-, and ethanol-induced gastric lesions in male NMRI mice.
Fasted male NMRI mice were randomly assigned into 12 groups (7 in each). The gastroprotective activity of vitamin B6 alone and in combination with L-cysteine and sodium hydrosulfate (NaHS) was evaluated against ethanol-, and indomethacin-induced gastric lesions. The animals were received vehicle, vitamin B6, L-cysteine, L-cysteine+vitamin B6, NaHS or NaHS+B6 before the induction of gastric lesions by ethanol (50%, 0.5 ml/25 g of body weight, orally) or indomethacin (40 mg/kg, orally). One and five hours after the administration of ethanol and indomethacin, respectively, the animals were sacrificed using anesthetics. The stomachs were removed, rinsed with normal saline and assessed for gastric wall mucus changes.
Pretreatment with L-cysteine, sodium hydrosulfate, and vitamin B6 significantly decreased the total area of gastric lesions (P<0.01). The mucus production in L-cysteine-, sodium hydrosulfate-, and vitamin B6-treated animals were significantly higher than in control rats P<0.05). The gastroprotective activity of L-cysteine and sodium hydrosulfate in combination with vitamin B6 were higher than when administered alone (P<0.05).
The result of this survey showed that the protective activity of L-cysteine and sodium hydrosulfate enhances in the presence of vitamin B6.
本研究旨在探讨维生素 B6（胱硫醚-γ 裂解酶和胱硫醚-β 合酶的辅助因子，也是 H2S 的前体）单独及与 L-半胱氨酸（H2S 的前体）联合应用对吲哚美辛和乙醇诱导的雄性 NMRI 小鼠胃损伤的保护作用。
禁食雄性 NMRI 小鼠随机分为 12 组（每组 7 只）。评估维生素 B6 单独及与 L-半胱氨酸和硫氢化钠（NaHS）联合应用对乙醇和吲哚美辛诱导的胃损伤的胃保护活性。动物在乙醇（50%，0.5ml/25g 体重，口服）或吲哚美辛（40mg/kg，口服）诱导胃损伤前接受载体、维生素 B6、L-半胱氨酸、L-半胱氨酸+维生素 B6、NaHS 或 NaHS+B6。在给予乙醇和吲哚美辛后 1 小时和 5 小时，分别用麻醉剂处死动物。取出胃，用生理盐水冲洗，评估胃壁黏液变化。
L-半胱氨酸、硫氢化钠和维生素 B6 预处理显著降低了胃损伤的总面积（P<0.01）。L-半胱氨酸、硫氢化钠和维生素 B6 处理动物的黏液生成量明显高于对照组大鼠（P<0.05）。L-半胱氨酸和硫氢化钠与维生素 B6 联合应用的胃保护活性高于单独应用时（P<0.05）。
本研究结果表明，L-半胱氨酸和硫氢化钠的保护活性在维生素 B6 存在时增强。
