Arikan Sedat, Ersan Ismail, Karaca Turan, Kara Selcuk, Gencer Baran, Karaboga Ihsan, Hasan Ali Tufan
Department of Ophthalmology, School of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Department of Histology and Embriyology, School of Medicine, Trakya University, Edirne, Turkey.
Arq Bras Oftalmol. 2015 Mar-Apr;78(2):100-4. doi: 10.5935/0004-2749.20150026.
This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina.
Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups.
The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 ± 5.6 µm and 16 ± 6.4 µm, respectively (P<0.05). Similarly, the mean thickness of ONL in the quercetin and ischemic groups was 50 ± 12.8 µm and 40 ± 8.7 µm, respectively (P<0.05). The antiapoptotic effect of quercetin in terms of reducing the numbers of both TUNEL (+) cells and caspase-3 (+) cells was significant in INL. The mean number of TUNEL (+) cells in INL in the ischemic and quercetin groups was 476.8 ± 45.6/mm2 and 238.72 ± 251/mm2, respectively (P<0.005). The mean number of caspase-3 (+) cells in INL of ischemic and quercetin groups was 633.6 ± 38.7/mm2 and 342.4 ± 36.1/mm2, respectively (P<0.001).
The use of quercetin may be beneficial in the treatment of retinal I/R injury because of its antiapoptotic effect on the retinal layers, particularly in INL.
本研究旨在探讨槲皮素对大鼠视网膜缺血再灌注（I/R）损伤诱导的凋亡性细胞死亡的影响。
将24只大鼠平均分为四组：对照组、缺血组、溶剂组和槲皮素组。通过将眼压升高至灌注压以上来实现I/R损伤。在I/R损伤前，分别对槲皮素组和溶剂组腹腔注射20mg/kg的槲皮素和二甲基亚砜（DMSO），然后让视网膜进行再灌注。损伤后48小时，测量所有组视网膜神经节细胞层（RGCL）、内核层（INL）、内网状层（IPL）、外网状层（OPL）和外核层（ONL）的厚度。此外，评估所有组INL和ONL中末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL(+)）细胞和半胱天冬酶-3(+)细胞的数量。
发现给予槲皮素可减少所有视网膜层的变薄。槲皮素组和缺血组INL的平均厚度分别为21±5.6μm和16±6.4μm（P<0.05）。同样，槲皮素组和缺血组ONL的平均厚度分别为50±12.8μm和40±8.7μm（P<0.05）。槲皮素在减少INL中TUNEL(+)细胞和半胱天冬酶-3(+)细胞数量方面的抗凋亡作用显著。缺血组和槲皮素组INL中TUNEL(+)细胞的平均数量分别为476.8±45.6/mm²和238.72±251/mm²（P<0.005）。缺血组和槲皮素组INL中半胱天冬酶-3(+)细胞的平均数量分别为633.6±38.7/mm²和342.4±36.1/mm²（P<0.001）。
由于槲皮素对视网膜层，特别是INL具有抗凋亡作用，其使用可能对治疗视网膜I/R损伤有益。
