Bae Jung Min, Jung Han Mi, Goo Boncheol, Park Young Min
Department of Dermatology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea.
Clinique L, Goyang, Korea.
Lasers Surg Med. 2015 Jul;47(5):433-40. doi: 10.1002/lsm.22358. Epub 2015 May 6.
Alopecia is one of the most common dermatological problems in the elderly; however, current therapies for it are limited by low efficacy and undesirable side effects. Although clinical reports on fractional laser treatment for various alopecia types are increasing, the exact mechanism remains to be clarified. The purposes of this study were to demonstrate the effect of ablative fractional laser treatment on hair follicle regrowth in vivo and investigate the molecular mechanism after laser treatment.
Ablative CO2 fractional laser was applied to the shaved dorsal skin of 7-week-old C57BL/6 mice whose hair was in the telogen stage. After 12 mice were treated at various energy (10-40 mJ/spot) and density (100-400 spots/cm(2) ) settings to determine the proper dosage for maximal effect. Six mice were then treated at the decided dosage and skin specimens were sequentially obtained by excision biopsy from the dorsal aspect of each mouse. Tissue samples were used for the immunohistochemistry and reverse transcription polymerase chain reaction assays to examine hair follicle status and their related molecules.
The most effective dosage was the 10 mJ/spot and 300 spots/cm(2) setting. The anagen conversion of hair was observed in the histopathological examination, while Wnt/β-catenin expression was associated with hair regrowth in the immunohistochemistry and molecular studies.
Ablative fractional lasers appear to be effective for inducing hair regrowth via activation of the Wnt/β-catenin pathway in vivo. Our findings indicate that fractional laser treatment can potentially be developed as new treatment options for stimulating hair regrowth.
脱发是老年人最常见的皮肤问题之一；然而，目前针对脱发的治疗方法疗效有限且存在不良副作用。尽管关于分次激光治疗各种类型脱发的临床报道不断增加，但其确切机制仍有待阐明。本研究的目的是证明剥脱性分次激光治疗对体内毛囊再生的影响，并研究激光治疗后的分子机制。
将剥脱性二氧化碳分次激光应用于7周龄处于休止期毛发的C57BL/6小鼠剃毛后的背部皮肤。对12只小鼠在不同能量（10 - 40 mJ/光斑）和密度（100 - 400光斑/cm²）设置下进行治疗，以确定产生最大效果的合适剂量。然后对6只小鼠按确定的剂量进行治疗，并通过切除活检从每只小鼠的背部依次获取皮肤标本。组织样本用于免疫组织化学和逆转录聚合酶链反应分析，以检查毛囊状态及其相关分子。
最有效的剂量是10 mJ/光斑和300光斑/cm²的设置。组织病理学检查观察到毛发的生长期转化，而免疫组织化学和分子研究表明Wnt/β-连环蛋白表达与毛发再生有关。
剥脱性分次激光似乎通过激活体内Wnt/β-连环蛋白途径有效诱导毛发再生。我们的研究结果表明，分次激光治疗有可能开发成为刺激毛发再生的新治疗选择。
