• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

生物大分子溶液多尺度散射的检测技术

Instrumentation on multi-scaled scattering of bio-macromolecular solutions.

作者信息

Chu Benjamin, Fang Dufei, Mao Yimin

机构信息

Chemistry Department, Stony Brook University, Stony Brook, New York, NY 11794-3400, USA.

出版信息

Int J Mol Sci. 2015 May 4;16(5):10016-37. doi: 10.3390/ijms160510016.

DOI:10.3390/ijms160510016
PMID:25946340
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4463630/
Abstract

The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light scattering (DLS), small angle X-ray scattering (SAXS) and wide angle X-ray diffraction (WAXD), the light scattering instrument is being developed to carry out studies in mildly turbid solutions, in the presence of multiple scattering. Three-dimensional photon cross correlation function (3D-PCCF) measurements have been introduced to couple with synchrotron X-ray scattering to study the structure, size and dynamics of macromolecules in solution.

摘要

介绍了一种结合激光光散射和同步加速器X射线散射的仪器的设计、构造及初步测试，该仪器可涵盖从埃量级的原子尺寸到微米的长度尺度研究，以及从微秒到秒的动力学研究。除了静态光散射（SLS）、动态光散射（DLS）、小角X射线散射（SAXS）和广角X射线衍射（WAXD）外，该光散射仪器正在开发中，以在存在多重散射的情况下对轻度浑浊溶液进行研究。三维光子互相关函数（3D-PCCF）测量已被引入，与同步加速器X射线散射相结合，以研究溶液中大分子的结构、大小和动力学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/c5c81e050138/ijms-16-10016-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/7e2b2c648d04/ijms-16-10016-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/070349013379/ijms-16-10016-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/da70b617137c/ijms-16-10016-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/bdca030ca8e8/ijms-16-10016-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/b50f7cc15adc/ijms-16-10016-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/dedcc14aa581/ijms-16-10016-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/55b60e06c65d/ijms-16-10016-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/10bd8dd4389d/ijms-16-10016-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/f58cb0b293a4/ijms-16-10016-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/344a86d7b432/ijms-16-10016-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/0677e5168c9d/ijms-16-10016-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/543d7b0490f2/ijms-16-10016-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/50f59b072bef/ijms-16-10016-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/857b218fca5b/ijms-16-10016-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/c5c81e050138/ijms-16-10016-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/7e2b2c648d04/ijms-16-10016-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/070349013379/ijms-16-10016-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/da70b617137c/ijms-16-10016-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/bdca030ca8e8/ijms-16-10016-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/b50f7cc15adc/ijms-16-10016-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/dedcc14aa581/ijms-16-10016-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/55b60e06c65d/ijms-16-10016-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/10bd8dd4389d/ijms-16-10016-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/f58cb0b293a4/ijms-16-10016-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/344a86d7b432/ijms-16-10016-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/0677e5168c9d/ijms-16-10016-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/543d7b0490f2/ijms-16-10016-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/50f59b072bef/ijms-16-10016-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/857b218fca5b/ijms-16-10016-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9558/4463630/c5c81e050138/ijms-16-10016-g015.jpg

相似文献

1
Instrumentation on multi-scaled scattering of bio-macromolecular solutions.生物大分子溶液多尺度散射的检测技术
Int J Mol Sci. 2015 May 4;16(5):10016-37. doi: 10.3390/ijms160510016.
2
Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12.多通道原位动态光散射仪器增强了在PETRA III光束线P12上进行的生物小角X射线散射实验。
J Synchrotron Radiat. 2018 Mar 1;25(Pt 2):361-372. doi: 10.1107/S1600577517017568. Epub 2018 Feb 13.
3
Structural Characterization of Biomaterials by Means of Small Angle X-rays and Neutron Scattering (SAXS and SANS), and Light Scattering Experiments.采用小角 X 射线和中子散射（SAXS 和 SANS）以及光散射实验对生物材料进行结构表征。
Molecules. 2020 Nov 29;25(23):5624. doi: 10.3390/molecules25235624.
4
Comprehensive characterization of nanostructured lipid carriers using laboratory and synchrotron X-ray scattering and diffraction.使用实验室和同步辐射 X 射线散射和衍射对纳米结构脂质载体进行综合表征。
Eur J Pharm Biopharm. 2019 Jun;139:153-160. doi: 10.1016/j.ejpb.2019.03.017. Epub 2019 Mar 21.
5
Atomic-resolution structural information from scattering experiments on macromolecules in solution.来自溶液中大分子散射实验的原子分辨率结构信息。
Phys Rev E Stat Nonlin Soft Matter Phys. 2013 May;87(5):052712. doi: 10.1103/PhysRevE.87.052712. Epub 2013 May 20.
6
[X-ray instrumentation for small-angle scattering].[用于小角散射的X射线仪器]
Tanpakushitsu Kakusan Koso. 1994 Aug;39(11):2003-10.
7
Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions.较小的毛细管可改善生物大分子溶液的小角X射线散射信号和样品消耗。
J Synchrotron Radiat. 2018 Jul 1;25(Pt 4):1113-1122. doi: 10.1107/S1600577518007907. Epub 2018 Jun 26.
8
A Mo-anode-based in-house source for small-angle X-ray scattering measurements of biological macromolecules.一种基于钼阳极的内部光源，用于生物大分子的小角X射线散射测量。
Rev Sci Instrum. 2016 Feb;87(2):025103. doi: 10.1063/1.4940936.
9
High Resolution Distance Distributions Determined by X-Ray and Neutron Scattering.高分辨率距离分布由 X 射线和中子散射确定。
Adv Exp Med Biol. 2017;1009:167-181. doi: 10.1007/978-981-10-6038-0_10.
10
Upgrade of MacCHESS facility for X-ray scattering of biological macromolecules in solution.用于溶液中生物大分子X射线散射的MacCHESS设施升级。
J Synchrotron Radiat. 2015 Jan;22(1):180-6. doi: 10.1107/S1600577514020360. Epub 2015 Jan 1.

本文引用的文献

1
Modulated 3D cross-correlation light scattering: improving turbid sample characterization.调制三维互相关光散射：改善浑浊样品表征
Rev Sci Instrum. 2010 Dec;81(12):123107. doi: 10.1063/1.3518961.
2
A new instrument for time-resolved static and dynamic light-scattering experiments in turbid media.一种用于在浑浊介质中进行时间分辨静态和动态光散射实验的新型仪器。
J Colloid Interface Sci. 2009 Aug 15;336(2):565-74. doi: 10.1016/j.jcis.2009.04.043. Epub 2009 Apr 21.
3
Structural classification of toxic amyloid oligomers.毒性淀粉样寡聚体的结构分类
J Biol Chem. 2008 Oct 31;283(44):29639-43. doi: 10.1074/jbc.R800016200. Epub 2008 Aug 22.
4
Dynamic light scattering in turbid nonergodic media.浑浊非遍历介质中的动态光散射
Rev Sci Instrum. 2008 Jul;79(7):073907. doi: 10.1063/1.2947756.
5
Effective suppression of multiply scattered light in static and dynamic light scattering.在静态和动态光散射中有效抑制多重散射光。
Appl Opt. 1998 Sep 20;37(27):6511-24. doi: 10.1364/ao.37.006511.
6
A beta oligomers - a decade of discovery.β-寡聚体——十年探索历程
J Neurochem. 2007 Jun;101(5):1172-84. doi: 10.1111/j.1471-4159.2006.04426.x. Epub 2007 Feb 5.
7
Multilayer vesicles and vesicle clusters formed by the fullerene-based surfactant C60(CH3)5K.由富勒烯基表面活性剂C60(CH3)5K形成的多层囊泡和囊泡簇。
J Colloid Interface Sci. 2004 Jul 15;275(2):632-41. doi: 10.1016/j.jcis.2004.02.048.
8
Small-angle scattering: a view on the properties, structures and structural changes of biological macromolecules in solution.小角散射：关于溶液中生物大分子的性质、结构及结构变化的见解
Q Rev Biophys. 2003 May;36(2):147-227. doi: 10.1017/s0033583503003871.
9
Multiple scattering suppression in static light scattering by cross-correlation spectroscopy.通过互相关光谱法抑制静态光散射中的多次散射
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1999 Nov;60(5 Pt B):5670-6. doi: 10.1103/physreve.60.5670.
10
Small-angle X-ray scattering of polymers.聚合物的小角X射线散射
Chem Rev. 2001 Jun;101(6):1727-61. doi: 10.1021/cr9900376.