Orct Tatjana, Lazarus Maja, Ljubojević Marija, Sekovanić Ankica, Sabolić Ivan, Blanuša Maja
Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia.
Biometals. 2015 Aug;28(4):701-12. doi: 10.1007/s10534-015-9859-3. Epub 2015 May 7.
Detoxification of mercury (Hg) with selenium (Se) in the early postnatal period with regard to the expression of metallothionein protein (MT), essential element status, and lipid peroxidation level in tissues has not been studied. Seven-day-old Wistar pups were orally pretreated with Se [6 μmol Na2SeO3/kg body weight (b.w.)] for 3 days and then cotreated with Hg (6 μmol HgCl2/kg b.w.) for the following 4 days. This group (Se + Hg) was compared to the groups treated with Hg, Se, or vehicle (control). Compared to the Hg-group, Se + Hg-group exhibited lower renal MT expression, reduced accumulation of Hg, Cu and Zn, and reduced excretion of Se, Hg and Zn in urine. In the liver, MT was stimulated by Se treatment in both, Se and Se + Hg-group. Hepatic and brain levels of the endogenous essential elements Cu, Fe, Mg, and Zn remained unchanged in all of the studied groups. Brain Hg levels and oxidation of lipids measured as thiobarbituric acid reactive substances were diminished in Se + Hg-group of pups compared to the Hg-group. This study suggests that Se pretreatment can help reduce Hg in the tissues of suckling rats, simultaneously preventing impairment of essential element levels in the kidneys and their excessive excretion via urine. Also, Se was shown to prevent oxidative damage of lipids in the brain, which is particularly susceptible to Hg during the early postnatal period.
关于出生后早期汞（Hg）与硒（Se）解毒作用对金属硫蛋白（MT）表达、必需元素状态以及组织中脂质过氧化水平的影响尚未进行研究。对7日龄的Wistar幼崽口服给予硒[6 μmol Na2SeO3/千克体重（b.w.）]预处理3天，然后在接下来的4天与汞（6 μmol HgCl2/千克b.w.）共同处理。将该组（Se + Hg）与用汞、硒或赋形剂（对照）处理的组进行比较。与汞组相比，Se + Hg组的肾脏MT表达较低，汞、铜和锌的积累减少，尿液中硒、汞和锌的排泄减少。在肝脏中，硒处理在硒组和Se + Hg组中均刺激了MT。在所有研究组中，内源性必需元素铜、铁、镁和锌的肝脏和大脑水平保持不变。与汞组相比，Se + Hg组幼崽的大脑汞水平以及以硫代巴比妥酸反应性物质衡量的脂质氧化减少。这项研究表明，硒预处理有助于降低乳鼠组织中的汞含量，同时防止肾脏中必需元素水平受损及其通过尿液过度排泄。此外，硒被证明可以防止大脑中脂质的氧化损伤，大脑在出生后早期对汞特别敏感。
