Sumi Shoichiro, Yanai Goichi
Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan,
Methods Mol Biol. 2015;1313:107-13. doi: 10.1007/978-1-4939-2703-6_7.
Auxiliary use of mesenchymal stem/stromal cells (MSCs) to islet transplantation is shown to enhance efficacy. We hypothesized cell fusion of islet cells and MSCs may provide a new cell source with robustness of MSCs and islet cell function. We succeeded electrofusion between dispersed islet cells and MSCs in rats and fused cells sustained beta-cell function in vitro and in vivo, suggesting their possibility of therapeutic application. Here, we describe our method of cell fusion that enabled us to fuse islet cells to MSCs.
间充质干/基质细胞（MSC）辅助胰岛移植可提高疗效。我们推测胰岛细胞与MSC的细胞融合可能会提供一种兼具MSC强大功能和胰岛细胞功能的新细胞来源。我们成功实现了大鼠分散胰岛细胞与MSC之间的电融合，且融合细胞在体外和体内均维持了β细胞功能，提示了其治疗应用的可能性。在此，我们描述了使我们能够将胰岛细胞与MSC融合的细胞融合方法。
