Fong Celesta, Dong Aurelia W, Hill Anita J, Boyd Ben J, Drummond Calum J
CSIRO Manufacturing Flagship, Private Bag 33, Clayton, VIC 3169, Australia.
Phys Chem Chem Phys. 2015 Jul 21;17(27):17527-40. doi: 10.1039/c5cp01921d.
Positron annihilation lifetime spectroscopy (PALS) has been shown to be highly sensitive to conformational, structural and microenvironmental transformations arising from subtle geometric changes in molecular geometry in self-assembling biomimetic systems. The ortho-positronium (oPs) may be considered an active probe that can provide information on intrinsic packing and mobility within low molecular weight solids, viscous liquids, and soft matter systems. In this perspective we provide a critical overview of the literature in this field, including the evolution of analysis software and experimental protocols with commentary upon the practical utility of PALS. In particular, we discuss how PALS can provide unique insight into the macroscopic transport properties of several porous biomembrane-like nanostructures and suggest how this insight may provide information on the release of drugs from these matrices to aid in developing therapeutic interventions. We discuss the potentially exciting and fruitful application of this technique to membrane dynamics, diffusion and permeability. We propose that PALS can provide novel molecular level information that is complementary to conventional characterisation techniques.
正电子湮没寿命谱（PALS）已被证明对自组装仿生系统中分子几何结构的细微变化所引起的构象、结构和微环境转变高度敏感。邻正电子素（oPs）可被视为一种活性探针，它能提供有关低分子量固体、粘性液体和软物质系统内部堆积和流动性的信息。从这个角度出发，我们对该领域的文献进行了批判性综述，包括分析软件和实验方案的发展，并对PALS的实际应用进行了评论。特别是，我们讨论了PALS如何能为几种多孔生物膜样纳米结构的宏观传输特性提供独特见解，并提出这种见解如何能提供有关药物从这些基质中释放的信息，以帮助开发治疗干预措施。我们讨论了该技术在膜动力学、扩散和渗透性方面潜在的令人兴奋且富有成果的应用。我们提出PALS可以提供与传统表征技术互补的新颖分子水平信息。
