支持珍珠鸡（Numida meleagris）代谢和免疫功能研究的分子工具。
Molecular tools to support metabolic and immune function research in the Guinea Fowl (Numida meleagris).
作者信息
Darris Carl E, Tyus James E, Kelley Gary, Ropelewski Alexander J, Nicholas Hugh B, Wang Xiaofei, Nahashon Samuel
机构信息
College of Agriculture, Human and Natural Sciences, Tennessee State University, Nashville, Tennessee, USA.
Pittsburgh Supercomputing Center, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
出版信息
BMC Genomics. 2015 May 7;16(1):358. doi: 10.1186/s12864-015-1520-6.
BACKGROUND
Guinea fowl (Numidia meleagris) production as an alternative source of meat and poultry has shown potential for economic viability. However, there has been little progress in characterizing the transcriptome of the guinea fowl. In this study RNA-sequencing and de novo transcriptome assembly of several Guinea fowl tissues (pancreas, hypothalamus, liver, bone marrow and bursa) which play key roles in regulating feed intake, satiety, and immune function was performed using Illumina's Hi-Seq 2000.
RESULTS
74 million sequences were generated and assembled into 96,492 contigs using the Trinity software suite. Over 39,000 of these transcripts were found to have in silico translated protein sequences that are homologous to chicken protein sequences. Gene ontology analysis uncovered 416 transcripts with metabolic functions and 703 with immune function.
CONCLUSION
The transcriptome information presented here will support the development of molecular approaches to improve production efficiency of the guinea fowl and other avian species.
背景
珍珠鸡（Numidia meleagris）养殖作为肉类和禽类的替代来源已显示出经济可行性潜力。然而，在表征珍珠鸡转录组方面进展甚微。在本研究中，使用Illumina的Hi-Seq 2000对在调节采食量、饱腹感和免疫功能中起关键作用的几种珍珠鸡组织（胰腺、下丘脑、肝脏、骨髓和法氏囊）进行了RNA测序和从头转录组组装。
结果
生成了7400万个序列，并使用Trinity软件套件组装成96492个重叠群。这些转录本中超过39000个具有与鸡蛋白质序列同源的计算机翻译蛋白质序列。基因本体分析发现416个具有代谢功能的转录本和703个具有免疫功能的转录本。
结论
本文提供的转录组信息将支持开发分子方法以提高珍珠鸡和其他禽类的生产效率。
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