Gao Yugang, Zhao Xueliang, Sun Chao, Zang Pu, Yang He, Li Ran, Zhang Lianxue
College of Traditional Chinese Medicine, Jilin Agricultural University, ChangChun, 130118, China.
College of AnimCal Science and Technology, Northwest A & F University, Yang Ling, 712100, China.
Virol J. 2015 May 7;12:73. doi: 10.1186/s12985-015-0301-9.
Bovine viral diarrhea virus (BVDV) infections are endemic in cattle populations worldwide and cause major economic losses. Thus, an effective vaccine is needed against the transmission of BVDV. The glycoprotein E(rns) is one of the envelope proteins of this virus and shows BVDV-related immunogenicity. Here, we report the use of Panax ginseng as an alternative production platform for the expression of glycoprotein E(rns) via Agrobacterium-mediated transformation.
Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR analyses showed that pBI121-E(rns) was stably integrated into the chromosome of transformants. ELISA assay and Western blot analysis confirmed the antigenicity of plant-derived E(rns) glycoprotein. Immunogenicity was evaluated subcutaneously in deer using a soluble protein extract of dried transgenic ginseng hairy roots. Specific humoral and cell-mediated immune responses against BVDV were detected following immunization.
These results demonstrated that the E(rns) glycoprotein could be expressed in ginseng hairy roots and that plant-derived glycoprotein E(rns) retained its antigenicity and immunogenicity.
牛病毒性腹泻病毒（BVDV）感染在全球牛群中呈地方性流行，并造成重大经济损失。因此，需要一种有效的疫苗来对抗BVDV的传播。糖蛋白E(rns)是该病毒的包膜蛋白之一，具有与BVDV相关的免疫原性。在此，我们报道了利用人参作为替代生产平台，通过农杆菌介导的转化来表达糖蛋白E(rns)。
聚合酶链反应（PCR）和逆转录（RT）-PCR分析表明，pBI121-E(rns)稳定整合到转化体的染色体中。酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析证实了植物源E(rns)糖蛋白的抗原性。使用干燥的转基因人参毛状根的可溶性蛋白提取物对鹿进行皮下免疫原性评估。免疫后检测到针对BVDV的特异性体液免疫和细胞介导免疫反应。
这些结果表明，E(rns)糖蛋白可在人参毛状根中表达，且植物源糖蛋白E(rns)保留了其抗原性和免疫原性。
