College of Traditional Material Medicine, Jinlin Agriculture University, Changchun, 130118, PR China.
Virol J. 2011 Feb 25;8:83. doi: 10.1186/1743-422X-8-83.
Bovine viral diarrhea virus (BVDV) infections continue to cause significantly losses in the deer population. Better isolation and identification of BVDV from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and control of BVDV. However, isolation and identification of BVDV from sika deer is seldom reported in literature. In this study, we collected some samples according to clinical sign of BVDV to isolation and identification of BVDV from sika deer.
we isolated a suspected BVDV strain from livers of an aborted fetus from sika deer in Changchun (China) using MDBK cell lines, named as CCSYD strain, and identified it by cytopathic effect (CPE), indirect immunoperoxidase test (IPX) and electron microscopy(EM). The results indicated that this virus was BVDV by a series of identification. The structural proteins E0 gene was cloned and sequenced. The obtained E0 gene sequence has been submitted to GenBank with the accession number: FJ555203. Alignment with other 9 strains of BVDV, 7 strains of classical swine fever virus (CSFV) and 3 strains of border disease virus(BDV) in the world, showed that the homology were 98.6%-84.8%, 76.0%-74.7%, 76.6%-77.0% for nucleotide sequence, respectively. The phylogenetic analysis indicated that new isolation and identification CCSYD strain belonged to BVDV1b.
To the best of our knowledge, this is the first report that BVDV was isolated and identified in sika deer. This current research contributes development new BVDV vaccine to prevent and control of BVD in sika deer.
牛病毒性腹泻病毒（BVDV）感染继续导致鹿群大量损失。更好地从梅花鹿中分离和鉴定 BVDV，可能会极大地促进预防性治疗和诊断试剂的开发，并有助于 BVDV 的预防和控制。然而，从梅花鹿中分离和鉴定 BVDV 的文献报道很少。在本研究中，我们根据 BVDV 的临床症状采集了一些样本，从长春（中国）的一只流产胎儿的肝脏中分离和鉴定了一种疑似 BVDV 株，命名为 CCSYD 株，并通过细胞病变效应（CPE）、间接免疫过氧化物酶试验（IPX）和电子显微镜（EM）进行了鉴定。结果表明，该病毒通过一系列鉴定被确认为 BVDV。克隆并测序了结构蛋白 E0 基因。获得的 E0 基因序列已提交给 GenBank，登录号为：FJ555203。与其他 9 株 BVDV、7 株经典猪瘟病毒（CSFV）和 3 株边界病病毒（BDV）在世界范围内的序列进行比对，发现核苷酸序列的同源性分别为 98.6%-84.8%、76.0%-74.7%、76.6%-77.0%。系统进化分析表明，新分离鉴定的 CCSYD 株属于 BVDV1b。
据我们所知，这是首次从梅花鹿中分离和鉴定 BVDV。本研究为开发预防和控制梅花鹿 BVD 的新型 BVDV 疫苗做出了贡献。
