Oates Caryn N, Külheim Carsten, Myburg Alexander A, Slippers Bernard, Naidoo Sanushka
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), Genomics Research Institute (GRI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Research School of Biology, Australian National University, 116 Daley Rd, Canberra, 0200, ACT, Australia.
Plant Cell Physiol. 2015 Jul;56(7):1418-28. doi: 10.1093/pcp/pcv064. Epub 2015 May 5.
Plants have evolved complex defenses that allow them to protect themselves against pests and pathogens. However, there is relatively little information regarding the Eucalyptus defensome. Leptocybe invasa is one of the most damaging pests in global Eucalyptus forestry, and essentially nothing is known regarding the molecular mechanisms governing the interaction between the pest and host. The aim of the study was to investigate changes in the transcriptional landscape and terpene profile of a resistant and susceptible Eucalyptus genotype in an effort to improve our understanding of this interaction. We used RNA-seqencing to investigate transcriptional changes following L. invasa oviposition. Expression levels were validated using real-time quantitative PCR. Terpene profiles were investigated using gas chromatography coupled to mass spectometry on uninfested and oviposited leaves. We found 698 and 1,115 significantly differentially expressed genes from the resistant and susceptible interactions, respectively. Gene Ontology enrichment and Mapman analyses identified putative defense mechanisms including cell wall reinforcement, protease inhibitors, cell cycle suppression and regulatory hormone signaling pathways. There were significant differences in the mono- and sesquiterpene profiles between genotypes and between control and infested material. A model of the interaction between Eucalyptus and L. invasa was proposed from the transcriptomic and chemical data.
植物已经进化出复杂的防御机制，使它们能够保护自己免受害虫和病原体的侵害。然而，关于桉树防御组的信息相对较少。桉树枝瘿姬小蜂是全球桉树林中最具破坏性的害虫之一，对于该害虫与宿主之间相互作用的分子机制基本上一无所知。本研究的目的是调查抗性和感性桉树基因型转录图谱和萜烯谱的变化，以增进我们对这种相互作用的理解。我们使用RNA测序来研究桉树枝瘿姬小蜂产卵后的转录变化。使用实时定量PCR验证表达水平。在未受侵染和已产卵的叶片上，使用气相色谱-质谱联用技术研究萜烯谱。我们分别从抗性和感性相互作用中发现了698个和1115个显著差异表达的基因。基因本体富集分析和Mapman分析确定了推定的防御机制，包括细胞壁强化、蛋白酶抑制剂、细胞周期抑制和调节激素信号通路。基因型之间以及对照和受侵染材料之间的单萜和倍半萜谱存在显著差异。根据转录组学和化学数据提出了桉树与桉树枝瘿姬小蜂相互作用的模型。
