Lim Sang Gu, Kim Young Soo, Han Hyung Kyun, Kim Kwang Suk, Kim Dae Jung, Kang Eon Jong, Son Sang Gyu, Kim Eung Oh
Inland Aquaculture Research Institute, NFRDI, Kyeongsangnam-do 645-806, Korea.
Dev Reprod. 2012 Dec;16(4):271-7. doi: 10.12717/DR.2012.16.4.271.
The effects of ovulation induction in ussurian bullhead, Leiocassis ussuriensis, were investigated by treating ussurian bullhead with hCG, LHRHa, GnRHa, ovaprim, and pimozide. hCG was injected to ussurian bullhead at 0.75% NaCl, 5,000, 10,000, 20,000, and 30,000 IU, respectively. The ovulation inducement rates were 100% in 20,000 and 30,000 IU. Fertilization rates were 82.7% and 79.8%. Hatching rates were 59.4% and 57.2%. Ovulation time was between 16-19 hr The concentrations of LHRHa injected were 0.75 NaCl, 50, 100, 200, 300, and 300 g/kg. The ovulation inducement rates were 100% in 300 and 400 µg/kg. Fertilization and hatching rates were 84.9% and 68.4% at 200 µg/kg. The times to ovulation were between 23 hr and 34 hr. Ovaprim of 0.75% NaCl, 1.0, 1.5, 2.0, 2.5 and 3.0 ml/kg were injected to the abdominal cavity. The ovulation inducement rate was highest at 2.0 and 3.0 ml/kg to 92% and ovulation time was between 27-38 hr. LHRHa concentrations of 0.75% NaCl, 50, 100, 200, 300 and 400 µg were injected with pimozide (1,000 µg). Ovulation inducement rate was 100% from 200 to 400 IU with pimozide. Ovulation time was 22-36 h. Fertilization and hatching rates were 88.9% and 70.4% in 200 µg/kg with pimozide.
通过用绒毛膜促性腺激素（hCG）、促黄体素释放激素类似物（LHRHa）、促性腺激素释放激素类似物（GnRHa）、奥维普利姆（ovaprim）和匹莫齐特（pimozide）处理乌苏里拟鲿，研究了排卵诱导对乌苏里拟鲿的影响。分别以0.75%氯化钠溶液、5000、10000、20000和30000国际单位的剂量向乌苏里拟鲿注射hCG。20000和30000国际单位组的排卵诱导率均为100%。受精率分别为82.7%和79.8%。孵化率分别为59.4%和57.2%。排卵时间在16 - 19小时之间。注射的LHRHa浓度分别为0.75%氯化钠溶液、50、100、200、300和400微克/千克。300和400微克/千克组的排卵诱导率为100%。200微克/千克组的受精率和孵化率分别为84.9%和68.4%。排卵时间在23小时至34小时之间。分别以0.75%氯化钠溶液、1.0、1.5、2.0、2.5和3.0毫升/千克的剂量向腹腔注射奥维普利姆。2.0和3.0毫升/千克组的排卵诱导率最高，为92%，排卵时间在27 - 38小时之间。将浓度为0.75%氯化钠溶液、50、100、200、300和400微克的LHRHa与匹莫齐特（1000微克）一起注射。匹莫齐特组200至400国际单位的排卵诱导率为100%。排卵时间为22 - 36小时。匹莫齐特组200微克/千克的受精率和孵化率分别为88.9%和70.4%。
