Animal Production and Fish Resources Department, Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt.
Theriogenology. 2012 May;77(8):1709-16. doi: 10.1016/j.theriogenology.2011.12.019. Epub 2012 Feb 25.
Nine groups each of four fish were injected with a single intramuscular dose of the following preparations: Physiological saline (0.9% NaCl) as a control group, 0.5 ml kg(-1) Ovaprim, 20 and 40 μg kg(-1) BW of GnRHa, 8 and 16 mL kg(-1) pimozide tablets and the following combination of GnRHa with pimozide (GP): 20 μg + 4 mg, 30 μg + 8 mg and 40 μg + 16 mg kg(-1) BW. The primary oocyte diameter (POD) before hormone administration ranged from 943.3 to 1071.0 μm. The latency periods (LP) were in the range of 9.0 to 12.0 h after injection. The highest ovulation ratio (OR) was observed in groups Ovaprim, GP(30 + 8) and GP(40 + 16). Other treatments were effective for ovulation, the ovulation ratio in Groups G(40) and GP(20 + 4) were significantly higher than G(20) treatment. The ovulation index (OI) was in the range 62 to 77% and showed significant differences among groups. There was no significant difference in fertilization ratio (FR) among Ovaprim, GP(30 + 8) and GP(40 + 16) groups, while there were significant difference between the previous group and G(20) and G(40) groups. Control, P8, P16 showed negative results in all the parameters LP, OED, OR, OI and FR. Levels of sex steroids were analyzed on 6 and 12 h after initiation of treatments. A significant increase in plasma E(2) with GP(30 + 8) injection was observed 6 and 12 h after injection, while there were no significant increase between all the other groups 6 h after injection. Treatments with GP(20 + 4) resulted in a significant increase in plasma T concentration in females compared with control after 6 h. In contrast, plasma T and E(2) concentrations were lower during the combined GP(20 + 4), GP(30 + 8) and GP(40 + 16) after 12 h than after 16 h of injection. The combined treatments (GnRHa + PIM) are better compared with Ovaprim which gave the same results, they have some advantages, such as reliable response and low cost. Ovaprim is more than 3 to 5-fold of the cost of (GnRH + PIM). Therefore, this method could be useful tool for commercial catfish breeders to ensure spawning success.
生理盐水（0.9%NaCl）作为对照组，0.5mlkg(-1)奥普瑞姆，20 和 40μgkg(-1)BW GnRHa，8 和 16mLkg(-1)匹莫齐特片和 GnRHa 与匹莫齐特（GP）的以下组合：20μg+4mg、30μg+8mg 和 40μg+16mgkg(-1)BW。激素给药前初级卵母细胞直径（POD）为 943.3 至 1071.0μm。潜伏期（LP）在注射后 9.0 至 12.0 小时之间。排卵率（OR）最高的是奥普瑞姆组、GP（30+8）和 GP（40+16）组。其他治疗方法对排卵有效，G（40）组和 GP（20+4）组的排卵率明显高于 G（20）治疗组。排卵指数（OI）在 62%至 77%之间，各组之间差异显著。奥普瑞姆、GP（30+8）和 GP（40+16）组之间的受精率（FR）无显著差异，而前一组与 G（20）和 G（40）组之间存在显著差异。对照组、P8、P16 在 LP、OED、OR、OI 和 FR 所有参数中均呈阴性结果。在治疗开始后 6 和 12 小时分析了性类固醇水平。GP（30+8）注射后 6 和 12 小时，血浆 E（2）水平显著升高，而其他所有组注射后 6 小时，血浆 E（2）水平均无显著升高。GP（20+4）治疗后，与对照组相比，6 小时后雌鱼血浆 T 浓度显著升高。相反，与注射后 16 小时相比，联合 GP（20+4）、GP（30+8）和 GP（40+16）后 12 小时血浆 T 和 E（2）浓度降低。与奥普瑞姆相比，联合治疗（GnRHa+PIM）效果更好，因为奥普瑞姆的成本是 GnRHa+PIM 的 3 到 5 倍以上。因此，这种方法可能是商业鲶鱼养殖户确保繁殖成功的有用工具。
