Oh Seong-Rip, Kang Hyeong-Cheol, Lee Chi-Hoon, Hur Sang-Woo, Lee Young-Don
Marine and Environmental Research Institute, Jeju National University, Jeju 695-965, Republic of Korea.
Dev Reprod. 2013 Jun;17(2):79-85. doi: 10.12717/DR.2013.17.2.079.
To understand the sex reversal characteristics in the longtooth grouper (Epinephelus bruneus), this study examined the sex reversal and artificial masculinization of wild caught E. bruneus reared in indoor rearing tank after a 17α-methyltestosterone injection. To domesticate a broodstock, 64 wild caught E. bruneus, between 47.0 to 110.0 cm in total length and from 1.5 to 21.4 kg in body weight, were reared in indoor rearing tank (4.0 to 5.0 m wide, and 2.5 to 3.0 m depth) for four years. Seven specimens showed sex reversal from female to male during indoor rearing condition, whose total length and body weights were from 63.0 to 99.0 cm and from 4.4 to 13.2 kg, respectively. After inducing artificial masculinization in 14 female E. bruneus with a 17α-methyltestosterone (2.0 mg/kg BW) implants for 3 years, spermiation occurred in 9 specimens (total length: 54.0 to 68.0 cm, body weight: 2.3 to 4.3 kg). Among the female to male sex reversals, two specimens returned back to being female, whose body weights were 2.8 kg (initially 2.6 kg) and 2.7 kg (initially 2.3 kg). Therefore, this study suggested that E. bruneus (> 3.0 kg) was more effective in masculinizing by 17α-methyltestosterone implants.
为了解斜带石斑鱼（Epinephelus bruneus）的性逆转特征，本研究对野生捕获的斜带石斑鱼在室内养殖池中饲养，并注射17α-甲基睾酮后，观察其性逆转和人工雄性化情况。为驯化亲鱼，将64尾野生捕获的斜带石斑鱼饲养在室内养殖池（宽4.0至5.0米，深2.5至3.0米）中四年，这些鱼的全长在47.0至110.0厘米之间，体重在1.5至21.4千克之间。在室内饲养条件下，有7尾鱼出现了从雌性到雄性的性逆转，其全长和体重分别为63.0至99.0厘米和4.4至13.2千克。在用17α-甲基睾酮（2.0毫克/千克体重）植入物对14尾雌性斜带石斑鱼进行人工雄性化诱导3年后，9尾鱼（全长：54.0至68.0厘米，体重：2.3至4.3千克）出现了排精现象。在雌性向雄性的性逆转中，有两尾鱼又变回了雌性，其体重分别为2.8千克（最初为2.6千克）和2.7千克（最初为2.3千克）。因此，本研究表明，体重超过3.0千克的斜带石斑鱼通过17α-甲基睾酮植入物进行雄性化更有效。
