Kim Ki Tae, Lim Han Kyu, Chang Young Jin
Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea.
Department of Fisheries and Marine Resources, Mokpo National University, Jeonnam 534-830, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):337-43. doi: 10.12717/devrep.2013.17.4.337.
Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at -1°C/min in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.
对太平洋牡蛎（Crassostrea gigas）解冻后的幼虫进行培育，以研究三种冷冻保存的幼虫随时间变化的存活率。解冻后幼虫的最高存活率和幼虫活力指数（LAI）来自于在-1°C/分钟的冷冻速度下，于0.2 M蔗糖和2.0 M乙二醇（EG）中渗透处理，刚解冻时担轮幼虫的存活率为63.8%、D形面盘幼虫为84.1%、早期壳顶幼虫为56.3%。在有食物供应的解冻后幼虫培育中，担轮幼虫存活至24小时、D形面盘幼虫存活至75小时、早期壳顶幼虫存活至57小时。结果表明，解冻后的各幼虫阶段均未进一步发育至后续相应阶段。
