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细胞黏附分子和明胶酶在人血清诱导的人眼睑来源干细胞体外聚集中的作用

A role of cell adhesion molecules and gelatinases in human serum-induced aggregation of human eyelid-derived stem cells in vitro.

作者信息

Yang Hyejin, Lim Yoon Hwa, Yun Sujin, Yoon A Young, Kim Haekwon

机构信息

Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.

出版信息

Dev Reprod. 2013 Dec;17(4):409-20. doi: 10.12717/DR.2013.17.4.409.

DOI:10.12717/DR.2013.17.4.409
PMID:25949157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4382947/
Abstract

Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of α2, α2B, αX, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against α2, α2B, and αX proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin α2 antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin α2, α2B, and αX, and MMP9 might play an important role in the HS-induced aggregation of HEACs.

摘要

据报道，在体外高密度培养期间，人血清（HS）可诱导人眼睑脂肪来源干细胞（HEACs）聚集。本研究聚焦于细胞黏附分子和明胶酶在HS诱导的HEACs聚集中的作用。HS诱导的聚集发生在培养的第9至15天。与HS培养基中未聚集的细胞（HS-ex）或对照胎牛血清培养的细胞相比，由HS培养基诱导聚集的细胞（HS-agg）显示出α2、α2B、αX和癌胚抗原相关细胞黏附分子1（CEACAM1）基因更强的表达。HS-agg被抗α2、α2B和αX蛋白的抗体清晰标记。蛋白质印迹结果表明，与HS-ex和对照胎牛血清培养的细胞相比，这两种整合素蛋白在HS-agg中大量表达。在HS培养基中培养HEACs期间用抗整合素α2抗体处理可延迟聚集体形成。与HS-ex或胎牛血清培养的细胞相比，HS-agg表现出基质金属蛋白酶1（MMP1）和基质金属蛋白酶9（MMP9）的强表达。与胎牛血清培养的条件培养基相比，HS培养的条件培养基显示MMP9明胶酶活性显著增加。然而，与HS诱导的聚集相关，金属蛋白酶组织抑制剂（TIMP）mRNA表达没有变化。基于这些结果，提示整合素α2、α2B、αX和MMP9可能在HS诱导的HEACs聚集中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/353dad1c9a1e/devrep-17-409-F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/ad79e19de152/devrep-17-409-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/3e0661d03530/devrep-17-409-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/042a51beff97/devrep-17-409-F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/388d95195457/devrep-17-409-F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/f0991b3570b9/devrep-17-409-F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/353dad1c9a1e/devrep-17-409-F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/ad79e19de152/devrep-17-409-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/3e0661d03530/devrep-17-409-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/042a51beff97/devrep-17-409-F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/388d95195457/devrep-17-409-F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/f0991b3570b9/devrep-17-409-F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/4382947/353dad1c9a1e/devrep-17-409-F6.jpg

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