Cha Sun-Yeong, Cheon Yong-Pil
Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Sungshin Women's University, Seoul 142-742, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):451-9. doi: 10.12717/DR.2013.17.4.451.
The biological activity of tissue specific stem cell is under the control of their specific microenvironment and the exogenous chemicals derived from digestive tract can be one of the constructing factors of that. It is suggested that the extract of brown algae Ishige okamurae has antioxidant-, apoptosis induction-, and antiinflammatory- effects. On the other hand, a few studies have shown that antioxidant assist inhibition of accumulation of fat. So we studied the effect of the extract of I. okamura on the cellular activity and differentiation of 3T3-L1 preadipocyte to adipose cell. The viability of cell was analyzed using 3-[4,5-dimethylthiazo-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. Adipogenesis of 3T3-L1 cell was analyzed after induction in the induction medium containing the I. okamurae extract. The cellular activity was high compared with the vehicle and 0.05 mM caffeine in all groups of I. okamurae extract treated cells. The extract of I. okamura inhibited accumulation of lipids in 10 and 50 μg/ml. The expression of the marker genes for adipocyte differentiation coincided with cytochemical results. These results suggest that the extract of I. okamurae increases the cellular viability of adipose precursor cells. On the other hand, it suppresses the differentiation of preadipocyte to adipocyte and accumulation of lipids in concentration-dependent manners. It may be possible that the major component of the extract can be applied in the control of adipose tissuegenesis.
组织特异性干细胞的生物活性受其特定微环境的控制，而源自消化道的外源性化学物质可能是该微环境的构成因素之一。有研究表明，冈村石莼提取物具有抗氧化、诱导凋亡和抗炎作用。另一方面，一些研究表明抗氧化剂有助于抑制脂肪堆积。因此，我们研究了冈村石莼提取物对3T3-L1前脂肪细胞向脂肪细胞的细胞活性和分化的影响。使用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑（MTT）法分析细胞活力。在含有冈村石莼提取物的诱导培养基中诱导后，分析3T3-L1细胞的脂肪生成。在所有经冈村石莼提取物处理的细胞组中，与载体和0.05 mM咖啡因相比，细胞活性较高。冈村石莼提取物在10和50μg/ml时可抑制脂质积累。脂肪细胞分化标记基因的表达与细胞化学结果一致。这些结果表明，冈村石莼提取物可提高脂肪前体细胞的细胞活力。另一方面，它以浓度依赖的方式抑制前脂肪细胞向脂肪细胞的分化和脂质积累。提取物的主要成分有可能应用于脂肪组织生成的控制。
