Walter S J, Zewde T, Shirley D G
Department of Physiology, Charing Cross and Westminster Medical School, London.
Q J Exp Physiol. 1989 Nov;74(6):805-12. doi: 10.1113/expphysiol.1989.sp003350.
Renal function was assessed in unrestrained conscious rats during either their active period (i.e. the hours of darkness) or their inactive period. On the following day, measurements were repeated after Inactin anaesthesia and preparation for clearance studies. In rats anaesthetized during their active period, preparation for clearance studies had no effect on inulin clearance (used as a measure of glomerular filtration rate), lithium clearance (used as an estimate of end-proximal fluid delivery) or fractional lithium excretion. In rats anaesthetized during their inactive period, the same procedures resulted in increases in all three variables, to reach values indistinguishable from those in animals studied during their active period. In both groups of rats there were increases in the fractional reabsorption of sodium and water in the distal nephron and in the urinary excretion of potassium. It is concluded that in anaesthetized rats prepared for clearance studies, rates of glomerular filtration and proximal tubular reabsorption (as indicated by lithium clearance) are similar to those in conscious animals during their active period.
在自由活动的清醒大鼠的活动期（即夜间时段）或非活动期评估其肾功能。次日，在注射戊巴比妥钠麻醉并准备进行清除率研究后重复测量。在活动期麻醉的大鼠中，准备进行清除率研究对菊粉清除率（用作肾小球滤过率的指标）、锂清除率（用作近端小管液流量的估计值）或锂排泄分数没有影响。在非活动期麻醉的大鼠中，相同的操作导致这三个变量均增加，达到与活动期研究的动物的值无显著差异的值。两组大鼠的远端肾单位钠和水的分数重吸收以及钾的尿排泄均增加。得出的结论是，在准备进行清除率研究的麻醉大鼠中，肾小球滤过率和近端小管重吸收率（如锂清除率所示）与清醒动物活动期的相似。
