Thomsen K, Olesen O V
Ren Physiol. 1981;4(4):165-72. doi: 10.1159/000172823.
The suitability of some commonly used experimental procedures for studying the effect of chronic alterations of sodium excretion on lithium clearance was examined in Wistar rats and Sprague-Dawley rats. The animals were maintained on diets with standardized sodium content for 10 days before the investigation. Urine flow and clearance values were measured in (1) awake unoperated rats, (2) awake catheterized rats and (3) anaesthetized rats of both strains. Awake unoperated and awake catheterized Wistar rats showed almost identical urine flows and clearances of sodium and lithium. Amytal anaesthetized Wistar rats showed significantly depressed urine flows and clearances of sodium, lithium and inulin as compared with awake rats. Awake unoperated and awake catheterized Sprague-Dawley rats showed almost identical clearance values but the urine flow rates were increased about fivefold in the awake catheterized Sprague-Dawley rats. Amytal anaesthetized catheterized Sprague-Dawley rats showed unchanged urine flows and clearance values as compared with awake rats. Inactin anaesthesia reduced the urine flows and clearance values in both Wistar rats and Sprague-Dawley rats. It is concluded that anaesthesia and surgery affect the kidney function to different degrees in different rat strains, amytal anaesthetized Sprague-Dawley rats being the least influenced. If exposure of the kidney is unnecessary, awake catheterized Wistar rats offer a suitable experimental procedure for determination of urine flow and urinary clearances of sodium and lithium.
在Wistar大鼠和Sprague-Dawley大鼠中，研究了一些常用实验方法对于研究钠排泄的慢性改变对锂清除率影响的适用性。在研究前，将动物维持在钠含量标准化的饮食上10天。测量了（1）清醒未手术大鼠、（2）清醒插管大鼠和（3）两种品系的麻醉大鼠的尿流量和清除率值。清醒未手术和清醒插管的Wistar大鼠显示出几乎相同的尿流量以及钠和锂的清除率。与清醒大鼠相比，戊巴比妥麻醉的Wistar大鼠显示出尿流量以及钠、锂和菊粉清除率显著降低。清醒未手术和清醒插管的Sprague-Dawley大鼠显示出几乎相同的清除率值，但清醒插管的Sprague-Dawley大鼠的尿流率增加了约五倍。与清醒大鼠相比，戊巴比妥麻醉的插管Sprague-Dawley大鼠显示尿流量和清除率值未改变。安泰酮麻醉降低了Wistar大鼠和Sprague-Dawley大鼠的尿流量和清除率值。得出的结论是，麻醉和手术在不同大鼠品系中对肾功能有不同程度的影响，戊巴比妥麻醉的Sprague-Dawley大鼠受影响最小。如果不需要暴露肾脏，清醒插管的Wistar大鼠为测定尿流量以及钠和锂的尿清除率提供了合适的实验方法。
