Ibrahim Dalia, Taniguchi Kazumi, Yamamoto Yoshio, Taniguchi Kazuyuki, Nakamuta Nobuaki
United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan.
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Microsc Res Tech. 2015 Jul;78(7):613-9. doi: 10.1002/jemt.22517. Epub 2015 May 6.
Little is known about the development of the olfactory organs of camel. In this study, prenatal development and neuronal differentiation of the vomeronasal organ (VNO) and the olfactory epithelium (OE) of the one-humped camel were studied by immunohistochemistry and lectin histochemistry. A neuronal marker, protein gene product (PGP) 9.5, but not a marker of fully differentiated olfactory receptor cells, olfactory marker protein, intensely labeled the olfactory receptor cells of the VNO and OE at 395 mm, 510 mm, and 530 mm fetal ages, indicating that the olfactory receptor cells are differentiated, but not fully matured both in the VNO and the OE. In 187 mm and 190 mm fetuses, PGP 9.5 yielded faint immunoreactive signals in the VNO, but not in the OE, although the presence of olfactory receptor cells were demonstrated in both tissues by intense WGA and LEL stainings. We conclude that the camel VNO and OE bear differentiated, but still immature receptor cells; in addition, the onset of neuronal differentiation seems to be somewhat earlier in the VNO than in the OE till half of the prenatal life.
关于骆驼嗅觉器官的发育，人们了解甚少。在本研究中，通过免疫组织化学和凝集素组织化学方法，对单峰骆驼犁鼻器（VNO）和嗅上皮（OE）的产前发育及神经元分化进行了研究。一种神经元标志物，即蛋白基因产物（PGP）9.5，而非完全分化的嗅觉受体细胞标志物嗅觉标记蛋白，在胎儿年龄为395毫米、510毫米和530毫米时，对VNO和OE的嗅觉受体细胞进行了强烈标记，这表明嗅觉受体细胞已分化，但在VNO和OE中均未完全成熟。在187毫米和190毫米的胎儿中，PGP 9.5在VNO中产生微弱的免疫反应信号，但在OE中未产生，尽管通过强烈的WGA和LEL染色在两种组织中均证实了嗅觉受体细胞的存在。我们得出结论，骆驼的VNO和OE具有已分化但仍未成熟的受体细胞；此外，直到产前生命的一半，VNO中神经元分化的开始似乎比OE中稍早。
