Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea.
Seowon Equine Clinic, Iljudong-ro 38, Jeju 63295, Republic of Korea.
Acta Histochem. 2021 Feb;123(2):151684. doi: 10.1016/j.acthis.2021.151684. Epub 2021 Jan 28.
We examined the localization of olfactory marker protein (OMP), protein gene product9.5 (PGP9.5), and glycan diversity in the vomeronasal organ (VNO) of the Korean black goat (Capra hircus coreanae) during the prenatal and postnatal periods using immunohistochemistry and lectin histochemistry. In fetal and 1-day-old goats, OMP was occasionally identified in receptor cells of the VNO sensory epithelium, and PGP9.5 was localized in both the sensory and non-sensory epithelia. In VNO from adult goats, OMP was abundant in the sensory epithelium and scarce in single cells of the non-sensory epithelium. These results suggest that OMP production is initiated in the VNO sensory epithelium (VNE) during the fetal stage, and that its activity is increased in adult VNO receptor cells and solitary cells in the non-sensory epithelium (VNSE). Furthermore, the free borders of the sensory epithelia were positive for 7 lectins, and 6 lectins were moderately and/or highly abundant in receptor cells. Supporting and basal cells, and nerve bundles had similar expression patterns. In VNE, 7 lectins were observed in the free border, and 6 in ciliated, goblet, and basal cells, and in gland acini. The intensities of WGA, LCA, and PNA were high in VSE receptor cells, and the intensity of PNA was high in ciliated cells of the VNSE. The other 3 lectins showed similar patterns throughout development. Collectively, these results confirm that the Korean black goat VNO starts developing during the late fetal stages and differentiates further after birth.
我们使用免疫组织化学和凝集素组织化学技术研究了韩国黑山羊（Capra hircus coreanae）在产前和产后期间鼻甲骨（VNO）中嗅觉标记蛋白（OMP）、蛋白基因产物 9.5（PGP9.5）和糖多样性的定位。在胎儿和 1 日龄山羊中，OMP 偶尔在 VNO 感觉上皮的受体细胞中被识别，PGP9.5 定位于感觉和非感觉上皮中。在成年山羊的 VNO 中，OMP 在感觉上皮中丰富，在非感觉上皮中的单个细胞中稀少。这些结果表明，OMP 的产生在胎儿期开始于 VNO 感觉上皮（VNE），并且其活性在成年 VNO 受体细胞和非感觉上皮（VNSE）中的单个细胞中增加。此外，感觉上皮的游离边界对 7 种凝集素呈阳性，6 种凝集素在受体细胞中中等和/或高度丰富。支持和基底细胞以及神经束具有相似的表达模式。在 VNE 中，游离边界观察到 7 种凝集素，在纤毛、杯状和基底细胞以及腺泡中观察到 6 种凝集素。WGA、LCA 和 PNA 在 VSE 受体细胞中的强度较高，PNA 在 VNSE 的纤毛细胞中的强度较高。其他 3 种凝集素在整个发育过程中表现出相似的模式。总的来说，这些结果证实韩国黑山羊的 VNO 在晚期胎儿期开始发育，并在出生后进一步分化。
