*Department of Pathology and Laboratory Medicine, Emilio Bernardelli Foundation, Casa di Cura San Carlo, Paderno Dugnano †Institute of Pathological Anatomy, University of Milan, Ospedale Luigi Sacco, Milano, Italy.
J Low Genit Tract Dis. 1999 Oct;3(4):254-9. doi: 10.1046/j.1526-0976.1999.34007.x.
To evaluate a possible mechanism of human immunodeficiency virus (HIV) and human papillomavirus (HPV) interaction, we have identified the cervical tissue compartments that harbor HIV.
We studied 39 paraffin-embedded, cervical conization specimens with high-grade cervical intraepithelial neoplasia (CIN3) occurring in HIV-infected women. From selected intraepithelial HPV-positive nonulcerated specimens (confirmed by in situ hybridization), we obtained serial 4- to 5-μm-thick sections that were stained with hematoxylin and eosin, anti-S100 protein, and anti-CD4. The presence of intramucosal Langerhans' cells or dendritic cells or CD4-positive cells was recorded. Three consecutive, nonmicrodissected, full-thickness sections of the same specimens were used for polymerase chain reaction (PCR) analysis (group A). Three other uncovered, consecutive sections from the same blocks were examined with an inverted microscope, and full-thickness specimens of mucosa were dissected from the underlying cervical stroma, were gently removed, and were used for PCR (group B). The quality of DNA was checked by HLA-DQa amplification; then a nested PCR for HIV proviral DNA was performed.
Of group A, 5 of 39 cases (12.8%) were positive, whereas HIV was not detected in the microdissected sections of group B, with or without intraepithelial Langerhans' or CD4 cells.
HIV does not affect cervical epithelium. The absence of infected Langerhans' or dendritic cells (or both) indicates a migration to the proximal lymph nodes of the in ….
为了评估人类免疫缺陷病毒（HIV）和人乳头瘤病毒（HPV）相互作用的可能机制，我们已经确定了携带 HIV 的宫颈组织隔室。
我们研究了 39 例石蜡包埋的宫颈锥切标本，这些标本均来自感染 HIV 的女性中发生的高级别宫颈上皮内瘤变（CIN3）。从选定的上皮内 HPV 阳性非溃疡性标本（通过原位杂交证实）中，我们获得了连续的 4-5μm 厚的切片，并用苏木精和曙红、抗 S100 蛋白和抗 CD4 染色。记录黏膜内朗格汉斯细胞或树突状细胞或 CD4 阳性细胞的存在情况。使用相同标本的 3 个连续的、非微切割的全层切片进行聚合酶链反应（PCR）分析（A 组）。从同一块的另外 3 个未覆盖的连续切片在倒置显微镜下检查，然后从下面的宫颈基质中轻轻切除黏膜的全层标本，并用于 PCR（B 组）。通过 HLA-DQa 扩增检查 DNA 的质量；然后进行 HIV 前病毒 DNA 的巢式 PCR。
A 组中，39 例中有 5 例（12.8%）为阳性，而 B 组中未在微切割切片中检测到 HIV，无论上皮内是否存在朗格汉斯细胞或 CD4 细胞。
HIV 不会影响宫颈上皮。感染的朗格汉斯细胞或树突状细胞（或两者）的缺失表明其迁移到近端淋巴结。
