人乳头瘤病毒基因型分布及人乳头瘤病毒 16 型和人乳头瘤病毒 18 型在人类免疫缺陷病毒感染妇女的浸润性宫颈癌及原位宫颈癌中的基因整合。
Human papillomavirus genotype distribution and human papillomavirus 16 and human papillomavirus 18 genomic integration in invasive and in situ cervical carcinoma in human immunodeficiency virus-infected women.
机构信息
Retrovirology Laboratory-IrsiCaixa Foundation, Badalona.
出版信息
Int J Gynecol Cancer. 2011 Nov;21(8):1486-90. doi: 10.1097/IGC.0b013e31822a0195.
BACKGROUND
Women infected with human immunodeficiency virus (HIV) are at increased risk of developing precancerous and cancerous lesions in cervix because of persistence of oncogenic human papillomavirus (HPV) infection. Scarce information about the HPV genotypes attributed to cervical cancer in the HIV-infected population is available, especially in countries with a low prevalence of this pathology.
OBJECTIVE
The objective of the study was to assess the prevalence and distribution of HPV types, and the viral integration of HPV-16 and HPV-18 in cervical squamous cell carcinoma of HIV-infected and HIV-negative women.
METHODS
A total of 140 formaldehyde-fixed paraffin-embedded specimens from 31 HIV-infected and 109 matched HIV-negative women, with a diagnosis of in situ or invasive cervical carcinoma, were identified between 1987 and 2010 from different hospitals of the Barcelona area, Spain. Human papillomavirus genotyping and integration were analyzed by standardized polymerase chain reaction.
RESULTS
Similar prevalence and distribution of HPV genotypes were detected in cervical cancers (in situ and invasive) regardless of HIV condition. The most common types were as follows: HPV-16 (58% in HIV-positive vs 72% in HIV-negative) and HPV-33 (16% vs 8%). In invasive cervical carcinoma, HPV-18 was significantly more prevalent in HIV-positive women (14% vs 1%; P = 0.014). The proportion of samples with integrated forms of HPV-16 (39% vs 45%) and HPV-18 (50% vs 50%) was similar in both groups.
CONCLUSIONS
The prevalence and distribution of principal HPV types involved in the carcinogenesis process of the cervix were similar in HIV-infected and noninfected women, although a tendency toward a lower HPV-16 and a higher HPV-18 prevalence in invasive cervical carcinoma was detected in HIV-positive women. Similar percentage of HPV-16 and HPV-18 viral integration was found in formaldehyde-fixed paraffin-embedded specimens of cervical cancer regardless of the HIV infection status.
背景
由于持续性致癌型人乳头瘤病毒（HPV）感染，感染人类免疫缺陷病毒（HIV）的女性发生宫颈癌前病变和癌症的风险增加。关于 HIV 感染者中与宫颈癌相关的 HPV 基因型的信息很少，特别是在该病理学发病率较低的国家。
目的
本研究旨在评估 HIV 感染和未感染妇女的宫颈鳞状细胞癌中 HPV 类型的流行率和分布情况，以及 HPV-16 和 HPV-18 的病毒整合情况。
方法
在西班牙巴塞罗那地区的不同医院，从 1987 年至 2010 年共确定了 31 例 HIV 感染和 109 例匹配的 HIV 阴性妇女的 140 例福尔马林固定石蜡包埋标本，这些妇女均诊断为原位或浸润性宫颈癌。采用标准化聚合酶链反应分析 HPV 基因分型和整合情况。
结果
无论 HIV 状况如何，宫颈癌（原位和浸润性）中 HPV 基因型的流行率和分布均相似。最常见的类型如下：HPV-16（58%在 HIV 阳性中，72%在 HIV 阴性中）和 HPV-33（16%在 HIV 阳性中，8%在 HIV 阴性中）。在浸润性宫颈癌中，HPV-18 在 HIV 阳性妇女中明显更为常见（14%对 1%；P=0.014）。HPV-16（39%对 45%）和 HPV-18（50%对 50%）整合形式的样本比例在两组中相似。
结论
在 HIV 感染和未感染妇女中，参与宫颈癌发生过程的主要 HPV 类型的流行率和分布相似，尽管在 HIV 阳性妇女中检测到 HPV-16 流行率降低和 HPV-18 流行率升高的趋势。在宫颈癌福尔马林固定石蜡包埋标本中，无论 HIV 感染状况如何，HPV-16 和 HPV-18 的病毒整合率相似。
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