Hiu I J
Laboratoire de Biochimie Extractive, CNRS AI 4310, Villejuif.
Ric Clin Lab. 1989 Jul-Sep;19(3):267-71. doi: 10.1007/BF02871816.
A 20 and 80% decrease of 3H-glucosamine incorporation was observed in resting human fibroblasts stimulated with fibroblast growth factor (FGF) and fetal calf serum (FCS), respectively. The decrease occurred as early as 15 min after stimulation. Inversely, DNA synthesis was increased 1 and 5 fold and cell number was increased 2 and 2.5 fold 72h later. 3H-glucosamine was incorporated in cell membrane glycopeptides (GP). Extracted from control or from cells exposed to FGF or FCS, GP have the same molecular weight and identical chemical constitution. The decrease of 3H-glucosamine incorporation caused by FGF and FCS appears to be related to the competitive binding between glucosamine-FGF and glucosamine-FCS components to GP, which probably constitute cell membrane receptors. A decline of 3H-glucosamine incorporation caused by FCS seems to result from the binding to cell-surface receptors (i.e., GP) of a large number of FCS components. The results suggest a correlation between cell growth rate and the density of growth-promoting molecules received by cells, since a large decrease of 3H-glucosamine incorporation corresponds to a high increase of DNA synthesis in cells exposed to FCS, which is a mixture of components with multiple effects.
在分别用成纤维细胞生长因子（FGF）和胎牛血清（FCS）刺激的静息人成纤维细胞中，观察到3H-葡萄糖胺掺入量分别下降了20%和80%。这种下降早在刺激后15分钟就出现了。相反，72小时后DNA合成增加了1倍和5倍，细胞数量增加了2倍和2.5倍。3H-葡萄糖胺掺入细胞膜糖肽（GP）中。从对照细胞或暴露于FGF或FCS的细胞中提取的GP具有相同的分子量和相同的化学组成。FGF和FCS引起的3H-葡萄糖胺掺入量的下降似乎与葡萄糖胺-FGF和葡萄糖胺-FCS成分与GP之间的竞争性结合有关，而GP可能构成细胞膜受体。FCS引起的3H-葡萄糖胺掺入量的下降似乎是由于大量FCS成分与细胞表面受体（即GP）结合所致。结果表明细胞生长速率与细胞接受的促生长分子密度之间存在相关性，因为在暴露于FCS的细胞中，3H-葡萄糖胺掺入量的大幅下降对应着DNA合成的大幅增加，而FCS是具有多种作用的成分的混合物。
