表面活性剂辅助提取果汁中的总酚含量（TPC）和抗氧化剂。

Surfactant mediated extraction of total phenolic contents (TPC) and antioxidants from fruits juices.

机构信息

Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160 014, India.

出版信息

Food Chem. 2015 Oct 15;185:284-8. doi: 10.1016/j.foodchem.2015.03.106. Epub 2015 Apr 1.

DOI:10.1016/j.foodchem.2015.03.106

Abstract

The aim of this study was to enhance the extraction of total phenolic contents (TPC) and antioxidants from fruit juices by the application of surfactants formulations instead of conventional solvents (methanol, ethanol and acetone). A variety of fruit infusions: apple red delicious (apple (rd)) (Malus domestica), Mcintosh apple (apple (i)) (Malus pumila), sweet lemon (Citrus limetta) and mango (Magnifera indica) were studied. Effect of water, organic solvents and five different aqueous surfactant formulations viz. SDS, Brij-35, Brij-58, Triton X-100 and Span-40 were explored for the extraction of TPC and determining the antioxidant activity (AA). The TPC and AA (%) were determined using Folin-Ciocalteu (FCA) and DPPH assay, respectively. The effect of surfactant type, concentration and common organic solvents on the extraction of TPC and AA (%) was studied using UV-visible spectrophotometric technique. Among all the extracting systems employed, Brij-58 showed the highest extraction efficiency.

摘要

本研究旨在通过应用表面活性剂配方代替传统溶剂（甲醇、乙醇和丙酮）来提高果汁中总酚含量（TPC）和抗氧化剂的提取效率。研究了多种水果浸提液：红富士苹果（苹果（rd））（Malus domestica）、麦金托什苹果（苹果（i））（Malus pumila）、甜柠檬（Citrus limetta）和芒果（Magnifera indica）。探讨了水、有机溶剂和五种不同的水相表面活性剂配方（SDS、Brij-35、Brij-58、Triton X-100 和 Span-40）对 TPC 提取和测定抗氧化活性（AA）的影响。采用福林-肖卡（FCA）和 DPPH 法测定 TPC 和 AA（%）。采用紫外可见分光光度法研究了表面活性剂类型、浓度和常见有机溶剂对 TPC 和 AA（%）提取的影响。在所使用的所有提取系统中，Brij-58 显示出最高的提取效率。