State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, No. 8 Da Yang Fang, Anwai, Chaoyang District, 100012, Beijing, China.
Environ Sci Pollut Res Int. 2015 Sep;22(18):13970-9. doi: 10.1007/s11356-015-4546-z. Epub 2015 May 9.
The moving bed biofilm reactors (MBBRs) were used to remove the residual NO3(-)-N of wastewater treatment plant (WWTP) effluent, and the MBBR carriers for denitrification were compared. The results showed that high denitrification efficiency can be achieved with polyethylene, polypropylene, polyurethane foam, and haydite carriers under following conditions: 7.2 to 8.0 pH, 24 to 26 °C temperature, 12 h hydraulic retention time (HRT), and 25.5 mg L(-1) external methanol dosage, while the WWTP effluent total nitrogen (TN) was between 2.6 and 15.4 mg L(-1) and NO3(-)-N was between 0.2 and 12.6 mg L(-1). The MBBR filled with polyethylene carriers had higher TN and NO3(-)-N removal rate (44.9 ± 19.1 and 83.4 ± 13.0%, respectively) than those with other carriers. The minimum effluent TN and NO3(-)-N of polyethylene MBBR were 1.6 and 0.1 mg L(-1), respectively, and the maximum denitrification rate reached 23.0 g m(-2) day(-1). When chemical oxygen demand (COD)/TN ratio dropped from 6 to 4, the NO3(-)- N and TN removal efficiency decreased significantly in all reactors except for that filled with polyethylene, which indicated that the polyethylene MBBR can resist influent fluctuation much better. The three-dimensional excitation-emission matrix analysis showed that all the influent and effluent of MBBRs contain soluble microbial products (SMPs)-like organics and biochemical oxygen demand (BOD), which can be removed better by MBBRs filled with haydite and polyethylene carriers. The nitrous oxide reductase (nosZ)-based terminal restriction fragment length polymorphism (T-RFLP) analysis suggested that the dominant bacteria in polyethylene MBBR are the key denitrificans.
移动床生物膜反应器 (MBBR) 被用于去除污水处理厂 (WWTP) 出水中的残留硝酸盐氮 (NO3(-)-N)，并对用于反硝化的 MBBR 载体进行了比较。结果表明，在以下条件下，聚乙烯、聚丙烯、聚氨酯泡沫和陶粒载体可实现高反硝化效率：pH 值为 7.2 至 8.0、温度为 24 至 26°C、水力停留时间 (HRT) 为 12 小时，外加甲醇剂量为 25.5 mg L(-1)，而 WWTP 出水总氮 (TN) 为 2.6 至 15.4 mg L(-1)，NO3(-)-N 为 0.2 至 12.6 mg L(-1)。填充聚乙烯载体的 MBBR 具有更高的 TN 和 NO3(-)-N 去除率（分别为 44.9 ± 19.1%和 83.4 ± 13.0%），高于其他载体。聚乙烯 MBBR 的最低出水 TN 和 NO3(-)-N 分别为 1.6 和 0.1 mg L(-1)，最大反硝化速率达到 23.0 g m(-2) day(-1)。当化学需氧量 (COD)/TN 比从 6 降至 4 时，除填充聚乙烯的反应器外，所有反应器的 NO3(-)-N 和 TN 去除效率均显著下降，这表明聚乙烯 MBBR 能更好地抵抗进水波动。三维激发-发射矩阵分析表明，MBBR 的所有进水和出水均含有类似于可溶微生物产物 (SMP) 的有机物和生化需氧量 (BOD)，而填充陶粒和聚乙烯载体的 MBBR 对其具有更好的去除效果。基于亚硝酸盐还原酶 (nosZ) 的末端限制性片段长度多态性 (T-RFLP) 分析表明，聚乙烯 MBBR 中的优势细菌是关键脱氮菌。
