Hashemi-Shahraki Abdolrazagh, Heidarieh Parvin, Biranvand Maryam, Bostanabad Saeed Zaker, Sheikhi Nasrin, Hashemzadeh Mohamad, Karami Masume, Feizabadi Mohammad Mehdi
Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Bacteriology and Immunology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Iran J Microbiol. 2014 Feb;6(1):1-7.
The increase of infections caused by nontuberculous mycobacteria (NTM) is receiving increasing attention worldwide. Mycobacterium fortuitum is encountered with increasing frequency in clinical laboratories of Iran.
Sequence variation of 48 M. fortuitum clinical isolates, were investigated by sequence analysis of the 16S-23S Internal Transcribed Spacer.
Twelve different sequence types (sequevar) were identified by sequence analysis of ITS region. Seven previously described sequevar including MfoA, MfoB, MfoC, MfoD, MfoE, MfoF and MfoG identified. Five novel sequevar namely MfoH, MfoI, MfoJ, MfoK and MfoL that were distinctly different from the previously described sequevar were detected among different clinical strains of M. fortuitum, from Iran.
This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level. The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran. The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.
非结核分枝杆菌（NTM）引起的感染增加在全球范围内日益受到关注。在伊朗的临床实验室中，偶然分枝杆菌的检出频率越来越高。
通过对16S - 23S内转录间隔区进行序列分析，研究了48株偶然分枝杆菌临床分离株的序列变异情况。
通过ITS区域序列分析鉴定出12种不同的序列类型（序列变种）。鉴定出7种先前描述的序列变种，包括MfoA、MfoB、MfoC、MfoD、MfoE、MfoF和MfoG。在来自伊朗的不同偶然分枝杆菌临床菌株中检测到5种新的序列变种，即MfoH、MfoI、MfoJ、MfoK和MfoL，它们与先前描述的序列变种明显不同。
本研究表明，ITS区域在临床分离株之间直至克隆水平具有很高的鉴别能力。结果还提示在伊朗受影响患者中可能存在偶然分枝杆菌的优势克隆。数据还得出结论，尽管某些克隆似乎占主导地位，但多种偶然分枝杆菌克隆均可致病。
