偶然分枝杆菌第三生物变种复合体的分类学变异：博尼克分枝杆菌新种、休斯顿分枝杆菌新种、新奥尔良分枝杆菌新种和布里斯班分枝杆菌新种的描述以及从人类临床分离株中鉴定出猪分枝杆菌。
Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.
作者信息
Schinsky Mark F, Morey Roger E, Steigerwalt Arnold G, Douglas Michael P, Wilson Rebecca W, Floyd Margaret M, Butler W Ray, Daneshvar Maryam I, Brown-Elliott Barbara A, Wallace Richard J, McNeil Michael M, Brenner Don J, Brown June M
机构信息
Washington University, School of Medicine, Barnes-Jewish Hospital, St Louis, MO 63110, USA.
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
出版信息
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1653-1667. doi: 10.1099/ijs.0.02743-0.
The Mycobacterium fortuitum third biovariant complex (sorbitol-negative and sorbitol-positive) contains unnamed taxa first characterized in 1991. These organisms can cause respiratory infections, a spectrum of soft tissue and skeletal infections, bacteraemia and disseminated disease. To evaluate this group of organisms, clinical reference isolates and the type strains of M. fortuitum third biovariant complex sorbitol-negative (n = 21), M. fortuitum third biovariant complex sorbitol-positive (n = 3), M. fortuitum (n = 3), Mycobacterium peregrinum (pipemidic acid-susceptible) (n = 1), Mycobacterium porcinum (n = 1), Mycobacterium senegalense (n = 2) and Mycobacterium septicum (n = 1) were characterized by using conventional phenotypic (morphological, physiological and antimicrobial susceptibilities), chemotaxonomic (HPLC and cellular fatty acids) and genotypic [RFLP of the rRNA gene (ribotyping), PCR-RFLP of a 439 bp segment of the 65 kDa hsp gene (PCR restriction analysis) and 16S rRNA gene sequence] analysis, DNA G + C content and DNA-DNA relatedness analyses. The results of these studies indicated that the strains comprised M. porcinum (n = 13), M. septicum (n = 1) and four novel closely related genetic groups within the M. fortuitum third biovariant complex: Mycobacterium boenickei sp. nov. (n = 6), Mycobacterium houstonense sp. nov. (n = 2), Mycobacterium neworleansense sp. nov. (n = 1) and Mycobacterium brisbanense sp. nov. (n = 1), with type strains ATCC 49935T (= W5998T = DSM 44677T), ATCC 49403T (= W5198T = DSM 44676T) ATCC 49404T (= W6705T = DSM 44679T) and ATCC 49938T (= W6743T = DSM 44680T), respectively.
偶然分枝杆菌第三生物变种复合体（山梨醇阴性和山梨醇阳性）包含1991年首次鉴定的未命名分类单元。这些微生物可引起呼吸道感染、一系列软组织和骨骼感染、菌血症及播散性疾病。为评估这组微生物，对临床参考菌株以及偶然分枝杆菌第三生物变种复合体山梨醇阴性（n = 21）、偶然分枝杆菌第三生物变种复合体山梨醇阳性（n = 3）、偶然分枝杆菌（n = 3）、海分枝杆菌（对吡哌酸敏感）（n = 1）、猪分枝杆菌（n = 1）、塞内加尔分枝杆菌（n = 2）和败血分枝杆菌（n = 1）的模式菌株进行了常规表型（形态学、生理学和抗菌药敏性）、化学分类学（高效液相色谱法和细胞脂肪酸分析）和基因型[核糖体RNA基因的限制性片段长度多态性分析（核糖体分型）、65 kDa热休克蛋白基因439 bp片段的聚合酶链反应-限制性片段长度多态性分析（PCR限制性分析）和16S核糖体RNA基因序列分析]分析、DNA G + C含量及DNA-DNA相关性分析。这些研究结果表明，这些菌株包括猪分枝杆菌（n = 13）、败血分枝杆菌（n = 1）以及偶然分枝杆菌第三生物变种复合体内四个新的密切相关的基因群：博氏分枝杆菌新种（n = 6）、休斯顿分枝杆菌新种（n = 2）、新奥尔良分枝杆菌新种（n = 1）和布里斯班分枝杆菌新种（n = 1），其模式菌株分别为ATCC 49935T（= W5998T = DSM 44677T）、ATCC 49403T（= W5198T = DSM 44676T）、ATCC 49404T（= W6705T = DSM 44679T）和ATCC 49938T（= W6743T = DSM 44680T）。
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