Xu Xiangqun, Teng Xiangshuai
Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Phys Med Biol. 2015 May 21;60(10):4185-95. doi: 10.1088/0031-9155/60/10/4185. Epub 2015 May 8.
Our previous work demonstrated that an optical coherence tomography (OCT) technique and the parameter 1/e light penetration depth (d1/e) were able to characterize the whole blood coagulation process in contrast to existing optical tests that are performed on plasma samples. To evaluate the feasibility of the technique for quantifying the effect of fibrinogen (Fbg) on blood coagulation, a dynamic study of d1/e of blood in various Fbg concentrations was performed in static state. Two groups of blood samples of hematocrit (HCT) in 35, 45, and 55% were reconstituted of red blood cells with: 1) treated plasma with its intrinsic Fbg removed and commercial Fbg added (0-8 g L(-1)); and 2) native plasma with commercial Fbg added (0-8 g L(-1)). The results revealed a typical behavior due to coagulation induced by calcium ions and the clotting time is Fbg concentration-dependent. The clotting time was decreased by the increasing amount of Fbg in both groups. Besides, the blood of lower HCT with various levels of Fbg took shorter time to coagulate than that of higher HCT. Consequently, the OCT method is a useful and promising tool for the detection of blood-coagulation processes induced with different Fbg levels.
我们之前的研究表明，与现有的针对血浆样本进行的光学检测相比，光学相干断层扫描（OCT）技术和参数1/e光穿透深度（d1/e）能够对全血凝血过程进行表征。为了评估该技术量化纤维蛋白原（Fbg）对血液凝固影响的可行性，在静态条件下对不同Fbg浓度血液的d1/e进行了动态研究。两组血细胞比容（HCT）分别为35%、45%和55%的血样，用以下方式重新构建红细胞：1）去除其固有Fbg并添加商业Fbg（0 - 8 g L(-1)）的处理后血浆；2）添加商业Fbg（0 - 8 g L(-1)）的天然血浆。结果显示了由钙离子诱导凝血的典型行为，且凝血时间依赖于Fbg浓度。两组中Fbg量增加均使凝血时间缩短。此外，不同Fbg水平下HCT较低的血液比HCT较高的血液凝血时间更短。因此，OCT方法是检测不同Fbg水平诱导的血液凝固过程的一种有用且有前景的工具。
