School of Science, Zhejiang Sci-Tech University, Hangzhou, China.
IEEE Trans Biomed Eng. 2012 Sep;59(9):2613-8. doi: 10.1109/TBME.2012.2207899. Epub 2012 Jul 11.
The feasibility of characterization of human blood fibrinogen levels using optical coherence tomography (OCT) was investigated. Three groups of blood samples were reconstituted of red blood cells: 1) phosphate-buffered saline; 2) plasma with its intrinsic fibrinogen removed and commercial fibrinogen added; and 3) native plasma with various fibrinogen levels (0-12 g/L). OCT signal slope (OCTSS) of blood was extracted from OCT depth-reflectivity profiles. Effects of hematocrit (HCT) and blood flow on OCTSS of the blood under various fibrinogen concentrations were also studied. The results of blood flowing at 5 mm/s showed that OCTSS of all the three groups at HCT of 40% decreases with the increasing fibrinogen concentration up to a certain level, i. e., >8, 6, and 4 g/L for groups 1, 2, and 3, respectively. The blood of group 2 at HCTs of 30%, 40%, and 50% had a rapid decrease in OCTSS in the range of fibrinogen concentration of 0-2, 0-6, and 0-10 g/L, respectively. OCTSS value of blood flowing at 2.5 mm/s was lower than that at 5 mm/s at each fibrinogen concentration. In conclusion, OCTSS has a strong correlation with plasma fibrinogen concentration, and has the potential to identify the abnormal level of human blood fibrinogen.
利用光相干断层扫描（OCT）对人血纤维蛋白原水平进行特征描述的可行性进行了研究。将三组血液样本用红细胞重构：1）磷酸盐缓冲盐水；2）去除血浆固有纤维蛋白原并添加商业纤维蛋白原；3）具有不同纤维蛋白原水平（0-12 g/L）的天然血浆。从 OCT 深度反射率曲线中提取血液的 OCT 信号斜率（OCTSS）。还研究了在不同纤维蛋白原浓度下，血细胞比容（HCT）和血流对血液 OCTSS 的影响。在 5 mm/s 血流速度下的结果表明，在 HCT 为 40%的情况下，三组血液的 OCTSS 均随纤维蛋白原浓度的增加而增加，直至一定水平，即分别为组 1、2 和 3 的>8、6 和 4 g/L。在 HCT 为 30%、40%和 50%的组 2 血液中，OCTSS 在纤维蛋白原浓度为 0-2、0-6 和 0-10 g/L 的范围内迅速下降。在每个纤维蛋白原浓度下，以 2.5 mm/s 流速流动的血液的 OCTSS 值均低于以 5 mm/s 流速流动的血液。总之，OCTSS 与血浆纤维蛋白原浓度有很强的相关性，有可能识别人血纤维蛋白原的异常水平。
