CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Bioresour Technol. 2015 Aug;190:251-6. doi: 10.1016/j.biortech.2015.04.052. Epub 2015 Apr 29.
Potential biohythane production from Sargassum sp. was evaluated in a two stage process. In the first stage, hydrogen dark fermentation was performed by Caldicellulosiruptor saccharolyticus. Sargassum sp. concentrations (VS) of 2.5, 4.9 and 7.4gL(-1) and initial inoculum concentrations (CDW) of 0.04 and 0.09gL(-1) of C. saccharolyticus were used in substrate/inoculum ratios ranging from 28 to 123. The end products from hydrogen production process were subsequently used for biogas production. The highest hydrogen and methane production yields, 91.3±3.3Lkg(-1) and 541±10Lkg(-1), respectively, were achieved with 2.5gL(-1) of Sargassum sp. (VS) and 0.09gL(-1)of inoculum (CDW). The biogas produced contained 14-20% of hydrogen. Potential energy production from Sargassum sp. in two stage process was estimated in 242GJha(-1)yr(-1). A maximum energy supply of 600EJyr(-1) could be obtained from the ocean potential area for macroalgae production.
采用两步法评估了马尾藻属（Sargassum sp.）生产生物沼气的潜力。在第一阶段，采用热纤梭菌（Caldicellulosiruptor saccharolyticus）进行氢气暗发酵。使用 2.5、4.9 和 7.4 gL(-1) 的马尾藻属（VS）浓度和 0.04 和 0.09 gL(-1) 的热纤梭菌初始接种物浓度（CDW），底物/接种物比范围为 28 至 123。氢气生产过程的最终产物随后用于沼气生产。当使用 2.5 gL(-1) 的马尾藻属（VS）和 0.09 gL(-1) 的接种物（CDW）时，获得了最高的氢气和甲烷产率，分别为 91.3±3.3 Lkg(-1) 和 541±10 Lkg(-1)。所产生的沼气中含有 14-20%的氢气。两步法从马尾藻属（Sargassum sp.）中估计的潜在能源产量为 242GJha(-1)yr(-1)。从海洋大型藻类生产的潜在区域，可获得最大 600EJyr(-1) 的能源供应。
