CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Bioresour Technol. 2016 Nov;219:132-138. doi: 10.1016/j.biortech.2016.07.096. Epub 2016 Jul 26.
Proof of principle of biohythane and potential energy production from garden waste (GW) is demonstrated in this study in a two-step process coupling dark fermentation and anaerobic digestion. The synergistic effect of using co-cultures of extreme thermophiles to intensify biohydrogen dark fermentation is demonstrated using xylose, cellobiose and GW. Co-culture of Caldicellulosiruptor saccharolyticus and Thermotoga maritima showed higher hydrogen production yields from xylose (2.7±0.1molmol(-1) total sugar) and cellobiose (4.8±0.3molmol(-1) total sugar) compared to individual cultures. Co-culture of extreme thermophiles C. saccharolyticus and Caldicellulosiruptor bescii increased synergistically the hydrogen production yield from GW (98.3±6.9Lkg(-1) (VS)) compared to individual cultures and co-culture of T. maritima and C. saccharolyticus. The biochemical methane potential of the fermentation end-products was 322±10Lkg(-1) (CODt). Biohythane, a biogas enriched with 15% hydrogen could be obtained from GW, yielding a potential energy generation of 22.2MJkg(-1) (VS).
本研究采用两步法，即暗发酵和厌氧消化相结合，从园林废物（GW）中生产生物丁烷和潜在能源，验证了其原理。本研究利用协同作用，使用极端嗜热菌共培养物来强化生物氢气暗发酵，以木糖、纤维二糖和 GW 作为发酵底物。与单一培养物相比，热纤维梭菌和海栖热袍菌的共培养物从木糖（2.7±0.1molmol(-1)总糖）和纤维二糖（4.8±0.3molmol(-1)总糖）中产生了更高的氢气产量。极端嗜热菌热纤维梭菌和卡尔迪克氏纤维菌的共培养物与单一培养物和海栖热袍菌与热纤维梭菌的共培养物相比，协同作用显著提高了 GW（98.3±6.9Lkg(-1)（VS））的氢气产量。发酵终产物的生物甲烷潜能为 322±10Lkg(-1)（CODt）。生物丁烷是一种富含 15%氢气的沼气，可以从 GW 中获得，产生 22.2MJkg(-1)（VS）的潜在能源。
