Wei Sheng Wu Xue Bao. 2015 Feb 4;55(2):205-13.
We studied the correlation between the microbial diversity in Panax notoginseng root soil and its root rot diseases, to find biological control approaches in Panax notoginseng soil borne diseases.
We isolated bacterial strains from the rhizosphere soils of healthy and root rot Panax notoginseng plants that are cultured continuously for 6 years in Wenshan Region. After separation and purification, we obtained DNA. On the basis of 16S rRNA' s general primer we carried out PCR amplification, conducted blast gene similarity and analyzed phylogenetic information.
The isolated bacterial strains distributed to 4 phyla, 40 genera of bacteria, 179 isolates from the samples of healthy Panax notoginseng rhizosphere soil belong to 30 genera and Burkholderia, Arthrobacter, Streptomyces and Bacillus are the dominant microflora. Additionally, 117 isolates from the samples of root rot Panax notoginseng rhizosphere soil belong to 29 genera and Ralstonia, Sphingomonas, Stenotrophomonas as the dominant microflora. Among them, Flavobacterium and Enterobacter were only isolated from the samples of root rot Panax notoginseng rhizosphere soil. At least 5 isolates are novel species; the ions concentration and electrical conductivity value show distinct discrepancy between the two groups (P < 0.05); the microbial amount of dominant species in the healthy soil samples present negative correlation with electrical conductivity value, the concentration of NO3-, SO4(2-), CO3(2-), K+ and total salt (P < 0.01).
In addition to pathogen infection, the physical and chemical characteristics, microbial community structure and the proportion of dominant species are also closely related to notoginseng continuous cropping soil borne disease. Especially the beneficial microorganisms (Burkholderia, Bacillus, Streptomyces, etc.) abundance is significant to evaluate the soil healthy condition and accurately disease control & forecast for Panax notoginseng cultivation.
研究三七根际土壤微生物多样性与其根腐病之间的相关性，以寻找三七土传病害的生物防治方法。
从文山地区连续种植6年的健康和根腐三七植株的根际土壤中分离细菌菌株。分离纯化后获得DNA。基于16S rRNA通用引物进行PCR扩增，进行基因相似性比对并分析系统发育信息。
分离出的细菌菌株分布于4个门、40个属，健康三七根际土壤样品中的179个分离株属于30个属，伯克霍尔德菌属、节杆菌属、链霉菌属和芽孢杆菌属为优势菌群。此外，根腐三七根际土壤样品中的117个分离株属于29个属，嗜麦芽窄食单胞菌属、鞘氨醇单胞菌属、嗜麦芽寡养单胞菌属为优势菌群。其中，黄杆菌属和肠杆菌属仅从根腐三七根际土壤样品中分离得到。至少有5个分离株为新物种；两组之间的离子浓度和电导率值存在明显差异（P<0.05）；健康土壤样品中优势种的微生物数量与电导率值、NO3-、SO4(2-)、CO3(2-)、K+浓度和总盐呈负相关（P<0.01）。
除病原菌感染外，理化性质、微生物群落结构和优势种比例也与三七连作土传病害密切相关。尤其是有益微生物（伯克霍尔德菌属、芽孢杆菌属、链霉菌属等）的丰度对于评估土壤健康状况以及准确防治和预测三七种植病害具有重要意义。
