Xu Gaihuan, Deng Linmei, Dao Jian, Wang Wenping, Liu Chunjiang, Yang Yanli, Zhao Jing, Liu Xia
Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, College of Plant Protection, Yunnan Agricultural University, Kunming, China.
Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming, China.
Front Plant Sci. 2025 Apr 28;16:1564704. doi: 10.3389/fpls.2025.1564704. eCollection 2025.
Soilborne pathogens significantly impact potato productivity by altering rhizosphere enzymatic activities and microbial communities. Pathogen-induced changes in enzyme activities are correlated with shifts in microbial community composition, but causal relationships remain unclear. This study investigates the effects of five key pathogens-, , , and -on soil enzyme activities and microbial community structure in potato rhizosphere soils under continuous cropping. This experiment involved pathogen inoculation and soil sampling in potato rhizosphere soils, with treatments replicated three times. Potatoes were planted on March 22, 2023, and harvested on August 25, 2023. Enzymatic activities were measured at different growth stages, and microbial communities were analyzed using high-throughput sequencing. Pathogen-induced variations in enzymatic activities were observed, potentially promoting disease proliferation. For instance, under stress, urease (URE) activity increased significantly at the full flowering and post-flowering stages, while catalase (CAT) activity decreased significantly during the seedling and full flowering stages. Under stress, activities of urease, sucrase (SUC), and alkaline phosphatase (ALP) decreased. stress led to a decline in URE and sucrase activities. infection led to a decrease in URE activity at the sowing stage. Furthermore, microbial community composition was significantly correlated with disease incidence, with specific taxa such as and showing negative correlations with incidence, while and were positively associated with . These results suggest that pathogen-induced changes in enzymatic activities play a critical role in disease dynamics and microbial interactions. The findings highlight the importance of understanding the effects of soilborne pathogens on soil enzyme activities and microbial communities, providing insights into disease management strategies in potato farming.
土传病原菌通过改变根际酶活性和微生物群落，对马铃薯生产力产生显著影响。病原菌诱导的酶活性变化与微生物群落组成的变化相关，但因果关系仍不明确。本研究调查了五种关键病原菌——、、、和——对连作条件下马铃薯根际土壤酶活性和微生物群落结构的影响。本实验包括在马铃薯根际土壤中接种病原菌和采集土壤样本，处理重复三次。马铃薯于2023年3月22日种植，2023年8月25日收获。在不同生长阶段测定酶活性，并使用高通量测序分析微生物群落。观察到病原菌诱导的酶活性变化，这可能促进病害增殖。例如，在胁迫下，脲酶（URE）活性在盛花期和盛花后期显著增加，而过氧化氢酶（CAT）活性在幼苗期和盛花期显著降低。在胁迫下，脲酶、蔗糖酶（SUC）和碱性磷酸酶（ALP）的活性降低。胁迫导致URE和蔗糖酶活性下降。感染导致播种期URE活性降低。此外，微生物群落组成与发病率显著相关，特定分类群如和与发病率呈负相关，而和与呈正相关。这些结果表明，病原菌诱导的酶活性变化在病害动态和微生物相互作用中起关键作用。研究结果突出了了解土传病原菌对土壤酶活性和微生物群落影响的重要性，为马铃薯种植中的病害管理策略提供了见解。
