[Antitumor mechanism of Bursopentin (BP5)].

OBJECTIVE

Bursopentin (BP5) is a multi-functional bioactive peptide with functions of immunomodulatory, antioxidant and antitumor. However, the antitumor mechanism of BP5 is still unclear.

METHODS

We constructed T7 phage cDNA library of DT40 cells, and the proteins interacted with BP5 were identified. Then, the expression profile of BP5-treated DT40 cells were analyzed using gene microarray, p53 Luciferase activity was detected.

RESULTS

The results of the expression profiling revealed that BP5 regulated expression of 1078 genes, of which 537 were up-regulated and 541 were down-regulated. Differentially expressed genes involved in various pathways were identified, of which 25 pathways were associated with immune responses and tumorigenic processes, including the p53 signaling. Furththmore, BP5 significantly enhanced p53 luciferase activity and stimulated expression of p53 protein in HCT116 cells.

CONCLUSION

These results suggest that BP5 exerted antitumor activity through p53 signaling and that this study provides novel insights on the antitumor mechanism of BP5.