Zhang Cong, Zhou Jiangfei, Li Shengnan, Cai Kairui, Guo Xiangling, Liao Chengshui, Wang Chen
Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, China.
Anticancer Agents Med Chem. 2018;18(11):1582-1588. doi: 10.2174/1871520618666180604094618.
The Bursa of Fabricius (BF) is acknowledged as the central humoral immune organ unique to birds. Bursal Hexapeptide (BHP, AGCCNG) is a recently reported bursal-derived bioactive peptide. However, there are few reports of the molecular basis of the mechanism on immune induction and potential antitumor activity of BHP.
In this paper, Gene microarray analyses demonstrated that BHP regulated expression of 1347 genes, of which 832 were up-regulated and 515 were down-regulated. Differentially expressed genes involved in various pathways were identified, of which 16 pathways were associated with immune responses and tumorigenic processes.
Specifically, we found that BHP selectively inhibited tumor cell proliferation. Furthermore, BHP enhanced antitumor factor p53 luciferase activity and stimulated expression of p53, p21, and p130 protein. Moreover, we observed that the inhibitory effect of BHP on cell proliferation and premature senescence in a p53-dependent manner.
Taken together, we uncovered that BHP may be involved in antitumor suppressor via p53 signaling pathway.
法氏囊（BF）被认为是鸟类特有的中枢体液免疫器官。法氏囊六肽（BHP，AGCCNG）是最近报道的一种源自法氏囊的生物活性肽。然而，关于BHP免疫诱导机制和潜在抗肿瘤活性的分子基础的报道很少。
本文通过基因芯片分析表明，BHP调节了1347个基因的表达，其中832个基因上调，515个基因下调。鉴定出参与各种途径的差异表达基因，其中16条途径与免疫反应和肿瘤发生过程相关。
具体而言，我们发现BHP选择性抑制肿瘤细胞增殖。此外，BHP增强了抗肿瘤因子p53荧光素酶活性，并刺激了p53、p21和p130蛋白的表达。此外，我们观察到BHP对细胞增殖和早衰的抑制作用以p53依赖的方式进行。
综上所述，我们发现BHP可能通过p53信号通路参与抗肿瘤抑制。
