Feng Zufei, Xu Yuehong, Wei Shuguang, Zhang Bao, Guan Fanglin, Li Shengbin
College of Forensic & Medicine, Xi'an Jiaotong University.
Anal Sci. 2015;31(5):399-406. doi: 10.2116/analsci.31.399.
A magnetic carbon nanomaterial for Fe3O4-modified hydroxylated multi-walled carbon nanotubes (Fe3O4-MWCNTs-OH) was prepared by the aggregating effect of Fe3O4 nanoparticles on MWCNTs-OH, and this material was combined with high-performance liquid chromatography (HPLC)/photodiode array detector (PAD) to determine strychnine in human serum samples. Some important parameters that could influence the extraction efficiency of strychnine were optimized, including the extraction time, amounts of Fe3O4-MWCNTs-OH, pH of sample solution, desorption solvent and desorption time. Under optimal conditions, the recoveries of spiked serum samples were between 98.3 and 102.7%, and the relative standard deviations (RSDs) ranged from 0.9 to 5.3%. The correlation coefficient was 0.9997. The LODs and LOQs of strychnine were 6.2 and 20.5 ng mL(-1), at signal-to-noise ratios of 3 and 10, respectively. These experimental results showed that the proposed method is feasible for the analysis of strychnine in serum samples.
通过Fe3O4纳米颗粒对羟基化多壁碳纳米管（Fe3O4-MWCNTs-OH）的聚集作用制备了一种磁性碳纳米材料，并将该材料与高效液相色谱（HPLC）/光电二极管阵列检测器（PAD）联用，用于测定人血清样品中的士的宁。优化了一些可能影响士的宁提取效率的重要参数，包括提取时间、Fe3O4-MWCNTs-OH用量、样品溶液pH值、解吸溶剂和解吸时间。在最佳条件下，加标血清样品的回收率在98.3%至102.7%之间，相对标准偏差（RSD）在0.9%至5.3%之间。相关系数为0.9997。士的宁的检测限和定量限分别为6.2和20.5 ng mL(-1)，信噪比分别为3和10。这些实验结果表明，所提出的方法可用于血清样品中士的宁分析。
