利用 HPLC 通过磁性分子印迹聚合物快速提取和检测酱油中的 4-甲基咪唑。
Fast Extraction and Detection of 4-Methylimidazole in Soy Sauce Using Magnetic Molecularly Imprinted Polymer by HPLC.
机构信息
Department of Applied Chemistry, School of Science, Xi'an University of Technology, Xi'an 710061, China.
School of Chemistry and Environmental Science, Lanzhou City University, Lanzhou 730070, China.
出版信息
Molecules. 2017 Nov 2;22(11):1885. doi: 10.3390/molecules22111885.
On the basis of magnetic molecularly imprinted polymer (MMIP) solid-phase extraction coupled with high performance liquid chromatography, we established a new method for the determination of the 4-methylimidazole (4-MEI) in soy sauce. Scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM) were used to characterize the synthesized MMIPs. To evaluate the polymers, batch rebinding experiments were carried out. The binding strength and capacity were determined from the derived Freundlich isotherm (FI) equation. The selective recognition capability of MMIPs was investigated with a reference compound and a structurally similar compound. As a selective pre-concentration sorbents for 4-methylimidazole in soy sauce, the MMIPs showed a satisfied recoveries rate of spiked samples, ranged from 97% to 105%. As a result, the prepared MMIPs could be applied to selectively pre-concentrate and determine 4-methylimidazole in soy sauce samples.
基于磁性分子印迹聚合物（MMIP）固相萃取与高效液相色谱法，我们建立了一种测定酱油中 4-甲基咪唑（4-MEI）的新方法。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和振动样品磁强计（VSM）对合成的 MMIP 进行了表征。为了评估聚合物，进行了批量结合实验。从衍生的弗伦德利希等温线（FI）方程中确定了结合强度和容量。通过参考化合物和结构相似的化合物研究了 MMIP 的选择性识别能力。作为酱油中 4-甲基咪唑的选择性预浓缩吸附剂，MMIPs 对加标样品的回收率令人满意，范围在 97%至 105%之间。因此，所制备的 MMIPs 可用于选择性预浓缩和测定酱油样品中的 4-甲基咪唑。
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