a Faculty of Science, Engineering and Technology , Swinburne University of Technology , Hawthorn , Australia.
Biofouling. 2015;31(3):297-307. doi: 10.1080/08927014.2015.1038706.
Aliphatic crystallites, characteristic of the eicosane and docosane components of naturally occurring lipids, were found to form microtextures that were structured by specific interactions with ordered graphite (HOPG) used as the underlying substratum, as confirmed by scanning electron microscopy (SEM) and fast Fourier transform (FFT) analysis. Confocal scanning laser microscopy (CLSM) showed highly directed bacterial alignment for two bacterial species (spherical and rod-shaped), reflecting the preferential orientation of the crystallite-air-water interfaces to give linear and triangular bacterial patterning. The mechanisms of bacterial attachment are demonstrated in terms of the balance between effective radial adhesional forces and the capillary forces resulting from the water contact angle of the bacteria at the three-phase line (TPL) of the lipid surface. It is suggested that these microtextured surfaces, which exhibit the ability to limit bacterial adhesion to a precise patterning at the lipid TPL, could be used as a means of controlling bacterial colonization.
脂族结晶，其特征为天然存在脂质的二十烷和二十二烷成分，被发现形成微纹理，这些微纹理通过与用作底层基底的有序石墨（HOPG）的特定相互作用而结构化，这一点已通过扫描电子显微镜 (SEM) 和快速傅里叶变换 (FFT) 分析得到证实。共焦扫描激光显微镜 (CLSM) 显示两种细菌（球形和杆形）具有高度定向的细菌排列，反映了结晶-空气-水界面的优先取向，从而形成线性和三角形的细菌图案。细菌附着的机制是根据有效径向粘附力与由于细菌在脂质三相线 (TPL) 处的水接触角而产生的毛细作用力之间的平衡来证明的。有人认为，这些具有微纹理的表面具有将细菌附着限制在脂质 TPL 的精确图案化的能力，可被用作控制细菌定植的手段。
