Savka Michael A, Dailey Lucas, Popova Milena, Mihaylova Ralitsa, Merritt Benjamin, Masek Marissa, Le Phuong, Nor Sharifah Radziah Mat, Ahmad Muhammad, Hudson André O, Bankova Vassya
The Thomas H. Gosnell School of Life Sciences, College of Science, Rochester Institute of Technology, 85 Lomb Memorial Dr., A350 Gosnell Hall, Rochester, NY 14623, USA.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Academic G. Bonchev Street, Building 9, 1113 Sofia, Bulgaria.
Evid Based Complement Alternat Med. 2015;2015:472593. doi: 10.1155/2015/472593. Epub 2015 Apr 15.
Propolis or bee glue has been used for centuries for various purposes and is especially important in human health due to many of its biological and pharmacological properties. In this work we showed quorum sensing inhibitory (QSI) activity of ten geographically distinct propolis samples from the United States using the acyl-homoserine lactone- (AHL-) dependent Chromobacterium violaceum strain CV026. Based on GC-MS chemical profiling the propolis samples can be classified into several groups that are as follows: (1) rich in cinnamic acid derivatives, (2) rich in flavonoids, and (3) rich in triterpenes. An in-depth analysis of the propolis from North Carolina led to the isolation and identification of a triterpenic acid that was recently isolated from Hondurian propolis (Central America) and ethyl ether of p-coumaric alcohol not previously identified in bee propolis. QSI activity was also observed in the second group US propolis samples which contained the flavonoid pinocembrin in addition to other flavonoid compounds. The discovery of compounds that are involved in QSI activity has the potential to facilitate studies that may lead to the development of antivirulence therapies that can be complementary and/or alternative treatments against antibiotic resistant bacterial pathogens and/or emerging pathogens that have yet to be identified.
蜂胶或蜂蜡已被用于各种目的几个世纪，由于其许多生物学和药理学特性，在人类健康方面尤为重要。在这项工作中，我们使用依赖于酰基高丝氨酸内酯（AHL）的紫色色杆菌菌株CV026，展示了来自美国的十个地理上不同的蜂胶样品的群体感应抑制（QSI）活性。基于气相色谱-质谱（GC-MS）化学分析，蜂胶样品可分为以下几组：（1）富含肉桂酸衍生物，（2）富含黄酮类化合物，（3）富含三萜类化合物。对来自北卡罗来纳州的蜂胶进行深入分析，导致分离和鉴定出一种三萜酸，该三萜酸最近从洪都拉斯蜂胶（中美洲）中分离出来，以及对香豆醇乙醚，这是以前在蜂蜂胶中未鉴定出的。在第二组美国蜂胶样品中也观察到QSI活性，这些样品除了含有其他黄酮类化合物外，还含有黄酮类化合物松属素。参与QSI活性的化合物的发现有可能促进相关研究，这些研究可能会导致开发出抗毒力疗法，这些疗法可以作为对抗抗生素耐药细菌病原体和/或尚未鉴定的新兴病原体的补充和/或替代治疗方法。
