Berhane Feven, Fite Alemu, Daboul Nour, Al-Janabi Wissam, Msallaty Zaher, Caruso Michael, Lewis Monique K, Yi Zhengping, Diamond Michael P, Abou-Samra Abdul-Badi, Seyoum Berhane
Wayne State University School of Medicine, Detroit, MI 48201, USA.
Georgia Regents University, Augusta, GA 30912, USA.
J Diabetes Res. 2015;2015:102054. doi: 10.1155/2015/102054. Epub 2015 Apr 19.
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.
胰岛素抵抗在2型糖尿病(T2D)发病机制中起核心作用,是预示T2D发生的早期指标。了解胰岛素抵抗早期细胞水平发生的代谢变化至关重要。本研究的目的是确定正常非糖尿病受试者口服葡萄糖耐量试验(OGTT)和高胰岛素正常血糖钳夹试验(HIEC)期间循环乳酸水平的变化模式。在22名参与者的OGTT和HIEC期间,每30分钟测定一次乳酸和甘油。在整个HIEC研究期间,乳酸水平逐渐升高(P < 0.001)。基线时,体重指数(BMI)< 30的参与者的平均M值显著高于BMI≥ 30的参与者(P < 0.05)。这种趋势在整个OGTT期间也持续存在。此外,糖耐量受损(IGT)者的平均乳酸水平显著高于糖耐量正常者(P < 0.001)。总之,我们发现HIEC研究期间乳酸水平升高,HIEC是一种高胰岛素血症状态,类似于T2D发生早期的代谢环境。