CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
J Infect. 2015 Sep;71(3):377-84. doi: 10.1016/j.jinf.2015.05.001. Epub 2015 May 9.
In France, cervical cancer screening is currently based on cytological examination of a Pap smear for women aged 25 to 65, but screening coverage is unsatisfactory. Previous studies have shown that self-sampling for human papillomavirus (HPV) testing increases rates of compliance. With this purpose in mind, we performed the CapU study to evaluate the acceptance of a urinary HPV test. Letters proposing a new cervical cancer screening method using at-home urine self-sampling were sent to 5000 women aged 40-65 years who had not had a Pap smear over the past three years. The participating patients had to send their urine samples to the Angers Hospital Virology Laboratory for analysis using real-time PCR. Of the 771 samples received, 687 were analyzed. High-risk HPV were detected in 29 women. In follow-up, 28 women with positive urinary HPV results had a Pap smear or colposcopy done. The cytological results showed nine abnormal Pap smears, among which histology studies confirmed three cases of cervical intraepithelial neoplasia grade III lesions. Our study shows that urinary HPV testing may be pertinent to women who do not have cervical Pap smears done and lead to the diagnosis of high-grade cervical lesions.
在法国，宫颈癌筛查目前基于对 25 至 65 岁女性的巴氏涂片细胞学检查，但筛查覆盖率不尽人意。先前的研究表明，人乳头瘤病毒（HPV）自我采样检测可提高依从率。基于此目的，我们进行了 CapU 研究，以评估尿 HPV 检测的可接受性。向过去三年未进行巴氏涂片检查的 5000 名 40-65 岁女性发送了关于使用家庭尿液自我采样进行新的宫颈癌筛查方法的建议信。参与的患者必须将尿液样本寄送到昂热医院病毒学实验室，使用实时 PCR 进行分析。在收到的 771 个样本中，有 687 个进行了分析。在 29 名女性中检测到高危型 HPV。在随访中，对 28 名尿液 HPV 检测阳性的女性进行了巴氏涂片或阴道镜检查。细胞学结果显示 9 例巴氏涂片异常，其中组织学研究证实了 3 例宫颈上皮内瘤变 III 级病变。我们的研究表明，尿液 HPV 检测可能适用于未进行宫颈巴氏涂片检查的女性，并可导致诊断出高级别宫颈病变。
