Zhong Zhi-Ping, Liu Ying, Liu Hong-Can, Wang Fang, Zhou Yu-Guang, Liu Zhi-Pei
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2653-2660. doi: 10.1099/ijs.0.000316. Epub 2015 May 11.
A Gram-stain-negative, catalase- and oxidase-positive, facultatively aerobic bacterium, strain DSG-S6T, was isolated from Dasugan Lake (salinity 3.1%, w/w), China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S6T were non-spore-forming, slightly bent rods, and motile by means of a single polar flagellum. Growth occurred in the presence of 0-7.0% (w/v) NaCl (optimum, 2.0%), at 4-35 °C (optimum, 30 °C) and at pH 6.0-10.5 (optimum, pH 8.0-8.5). C16 : 0, C18 : 1ω7c and C16 : 1ω7c and/or C16 : 1ω6c were the major fatty acids. Six alleles of the 16S rRNA gene sharing 98.9-99.9 % similarity were detected in strain DSG-S6T, which showed highest 16S rRNA gene sequence similarity to Vibrio aestuarianus ATCC 35048T (97.7 %), then to Vibrio pacinii LMG 19999T (97.6%) and Vibrio metschnikovii CIP 69.14T (96.8%). Multilocus sequence analysis of four housekeeping genes and 16S rRNA genes clearly clustered it as a member of the Anguillarum clade. Mean DNA-DNA relatedness between strain DSG-S6T and V. aestuarianus NBRC 15629T, V. pacinii CGMCC 1.12557T and V. metschnikovii JCM 21189T was 20.6 ± 2.3, 38.1 ± 3.5 and 24.2 ± 2.8%, respectively. The DNA G+C content was 46.8 mol% (Tm). Based on the data, it is concluded that strain DSG-S6T represents a novel species of the genus Vibrio, for which the name Vibrio salilacus sp. nov. is proposed. The type strain is DSG-S6T ( = CGMCC 1.12427T = JCM 19265T).
从中国达苏干湖（盐度3.1%，w/w）分离出一株革兰氏染色阴性、过氧化氢酶和氧化酶阳性、兼性需氧细菌，菌株DSG-S6T。采用多相分类法确定其分类地位。菌株DSG-S6T的细胞不形成芽孢，呈微弯杆状，借助单根极生鞭毛运动。在0-7.0%（w/v）NaCl（最适浓度为2.0%）、4-35 °C（最适温度为30 °C）和pH 6.0-10.5（最适pH为8.0-8.5）条件下生长。C16 : 0、C18 : 1ω7c和C16 : 1ω7c和/或C16 : 1ω6c是主要脂肪酸。在菌株DSG-S6T中检测到16S rRNA基因的6个等位基因，相似性为98.9-99.9%，其16S rRNA基因序列与河口弧菌ATCC 35048T的相似性最高（97.7%），其次是帕氏弧菌LMG 19999T（97.6%）和梅氏弧菌CIP 69.14T（96.8%）。对四个管家基因和16S rRNA基因进行多位点序列分析，结果明确将其聚类为鳗弧菌属的一个成员。菌株DSG-S6T与河口弧菌NBRC 15629T、帕氏弧菌CGMCC 1.12557T和梅氏弧菌JCM 21189T的平均DNA-DNA相关性分别为20.6 ± 2.3%、38.1 ± 3.5%和24.2 ± 2.8%。DNA G+C含量为46.8 mol%（Tm）。根据这些数据，得出结论：菌株DSG-S6T代表弧菌属的一个新物种，为此提出新名称盐生弧菌（Vibrio salilacus sp. nov.）。模式菌株为DSG-S6T（=CGMCC 1.12427T =JCM 19265T）。
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