State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
Virology. 2015 Sep;483:54-63. doi: 10.1016/j.virol.2015.03.039. Epub 2015 May 15.
Peste des petits ruminants virus (PPRV) causes a fatal disease in small ruminants. V protein of PPRV plays a pivotal role in interfering with host innate immunity by blocking IFNs signaling through interacting with STAT1 and STAT2. In the present study, the results demonstrated that PPRV V protein blocks IFN actions in a dose dependent manner and restrains the translocation of STAT1/2 proteins. We speculate that the translocation inhibition might be caused by the interfering of the downstream of STAT protein. Mutagenesis defines that Cys cluster and Trp motif of PPRV V protein are essential for STAT-mediated IFN signaling. These findings give a new sight for the further studies to understand the delicate mechanism of PPRV to escape the IFN signaling.
小反刍兽疫病毒（PPRV）可导致小反刍动物发生致命性疾病。PPRV 的 V 蛋白通过与 STAT1 和 STAT2 相互作用阻断 IFN 信号通路，在干扰宿主固有免疫方面发挥关键作用。本研究结果表明，PPRV V 蛋白以剂量依赖的方式阻断 IFN 的作用，并抑制 STAT1/2 蛋白的易位。我们推测这种易位抑制可能是由 STAT 蛋白下游的干扰引起的。突变分析表明，PPRV V 蛋白的半胱氨酸簇和色氨酸基序对于 STAT 介导的 IFN 信号转导至关重要。这些发现为进一步研究 PPRV 逃避 IFN 信号转导的精细机制提供了新的视角。
