铜绿假单胞菌分离株中 Pf1 样（前）噬菌体遗传元件的流行情况。

Prevalence of Pf1-like (pro)phage genetic elements among Pseudomonas aeruginosa isolates.

机构信息

Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Vojvodina, Serbia.

Laboratory of Gene Technology, Department Biosystems, KU Leuven, Kasteelpark Arenberg 21 box 2462, B-3001 Leuven, Belgium.

出版信息

Virology. 2015 Sep;483:64-71. doi: 10.1016/j.virol.2015.04.008. Epub 2015 May 15.

DOI:10.1016/j.virol.2015.04.008

PMID:25965796

Abstract

Pf1-like bacteriophages (family Inoviridae) of Pseudomonas aeruginosa can contribute to bacterial short term evolution and virulence. Here we examine Pf1-like (pro)phage diversity and prevalence among different P. aeruginosa isolates. Pf1-like prophages in sequenced genomes of P. aeruginosa were analyzed and grouped into four clades: Pf4, Pf5, Pf7 and Pf-LES. P. aeruginosa strains (n=241) were screened for the presence of universal (primers PfUa and PfUb) and specific Pf1-like genetic elements (Pf1, Pf4 and Pf5). More than half of the strains contained at least one Pf1-like genetic element (60%); universal elements were detected in 56% of the strains, Pf4 in 22%, Pf1 in 18% and Pf5 in 7%. Infectivity experiments confirmed that strains yielding PCR products with either universal or Pf4 specific primers can release infective virions. Based on the high prevalence of Pf1-like (pro)phages, it is necessary to further examine their involvement in P. aeruginosa virulence.

摘要

假单胞菌 Pf1 样噬菌体（Inoviridae 科）可促进细菌短期进化和毒力。本研究旨在检测不同铜绿假单胞菌分离株中 Pf1 样（前）噬菌体的多样性和流行情况。对铜绿假单胞菌测序基因组中的 Pf1 样前噬菌体进行分析，并分为 4 个进化枝：Pf4、Pf5、Pf7 和 Pf-LES。筛选 241 株铜绿假单胞菌以检测通用（引物 PfUa 和 PfUb）和特定 Pf1 样遗传元件（Pf1、Pf4 和 Pf5）的存在。超过一半的菌株至少含有一个 Pf1 样遗传元件（60%）；56%的菌株检测到通用元件，22%的菌株检测到 Pf4，18%的菌株检测到 Pf1，7%的菌株检测到 Pf5。感染性实验证实，用通用或 Pf4 特异性引物产生 PCR 产物的菌株可以释放感染性病毒粒子。鉴于 Pf1 样（前）噬菌体的高流行率，有必要进一步研究它们在铜绿假单胞菌毒力中的作用。