Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan.
Arch Virol. 2012 Apr;157(4):733-8. doi: 10.1007/s00705-011-1210-x. Epub 2012 Jan 5.
Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infecting P. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
噬菌体现已被应用于治疗铜绿假单胞菌感染的实验性噬菌体疗法中。为了研究噬菌体 KPP10 作为治疗性噬菌体的适用性，对其基因组进行了分析。基因组 DNA 长 88322bp，有 158 个开放阅读框（ORF），预测有三个 tRNA 基因。未预测到 ORF 编码的致病性或溶原性因子。比较基因组分析表明，噬菌体 KPP10 与噬菌体 PAK_P3 可以归为感染铜绿假单胞菌的新型裂解噬菌体。由于噬菌体 KPP10 是一种没有 ORF 编码的致病性或溶原性因子的裂解噬菌体，因此被认为适合用于治疗目的。
